Transcriptional Profiling and Transposon Mutagenesis Study of the Endophyte Pantoea eucalypti FBS135 Adapting to Nitrogen Starvation
Abstract
:1. Introduction
2. Results and Analysis
2.1. FBS135 Transcriptomes of High Quality Were Obtained from Conditions with the Presence and Absence of NH4+
2.2. P. eucalypti FBS135 Modulates Its Gene Transcription Adapting to Nitrogen Scarcity
2.3. Nitrogen Utilization-Related Genes of P. eucalypti FBS135 Were Significantly Downregulated in Expression upon Nitrogen Starvation
2.4. sRNAs Were Predicted with Potential Roles in the Adaptation to Nitrogen Starvation
2.5. Five Tn5 Insertion Mutants of P. eucalypti FBS135 Were Identified to Be Associated with Nitrogen Utilization
2.6. Functional Analysis of the Tn5-Disrupted Genes Revealed Their Potential in the Adaption of P. eucalypti FBS135 to Nitrogen Scarcity
3. Discussion
4. Materials and Methods
4.1. Strain Materials and Growth Conditions
4.2. RNA Sequencing
4.3. Transcriptome Analysis
4.4. Quantitative PCR
4.5. Construction of the Mutant Library
4.6. Determination of the Gene Locations of Tn5 Transposon
4.7. Verification of the Locations of Tn5 Transposon
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample Name | Raw Reads | Clean Reads | Clean Bases | Error Rate | Q 20 | Q 30 | GC Content |
---|---|---|---|---|---|---|---|
AN_WT_1 | 7,797,428 | 7,673,596 | 1.16G | 0.02 | 98.27 | 94.73 | 55.31 |
AN_WT_2 | 7,208,796 | 7,091,792 | 1.07G | 0.02 | 98.37 | 94.99 | 53.84 |
AN_WT_3 | 7,801,876 | 7,697,252 | 1.16G | 0.02 | 98.48 | 95.19 | 52.76 |
A_WT_1 | 7,637,382 | 7,550,282 | 1.14G | 0.02 | 98.14 | 94.33 | 50.37 |
A_WT_2 | 7,858,328 | 7,767,674 | 1.17G | 0.02 | 98.16 | 94.42 | 51.5 |
A_WT_3 | 7,435,342 | 7,338,626 | 1.11G | 0.02 | 98.36 | 94.94 | 51.66 |
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Huang, S.; Zhang, X.; Song, Z.; Rahman, M.U.; Fan, B. Transcriptional Profiling and Transposon Mutagenesis Study of the Endophyte Pantoea eucalypti FBS135 Adapting to Nitrogen Starvation. Int. J. Mol. Sci. 2023, 24, 14282. https://doi.org/10.3390/ijms241814282
Huang S, Zhang X, Song Z, Rahman MU, Fan B. Transcriptional Profiling and Transposon Mutagenesis Study of the Endophyte Pantoea eucalypti FBS135 Adapting to Nitrogen Starvation. International Journal of Molecular Sciences. 2023; 24(18):14282. https://doi.org/10.3390/ijms241814282
Chicago/Turabian StyleHuang, Shengquan, Xiuyu Zhang, Zongwen Song, Mati Ur Rahman, and Ben Fan. 2023. "Transcriptional Profiling and Transposon Mutagenesis Study of the Endophyte Pantoea eucalypti FBS135 Adapting to Nitrogen Starvation" International Journal of Molecular Sciences 24, no. 18: 14282. https://doi.org/10.3390/ijms241814282