Transcriptomic and Metabolomic Analyses Reveal the Key Genes Related to Shade Tolerance in Soybean
Abstract
:1. Introduction
2. Results
2.1. Physiological Characteristics of Soybean Leaves under Different Shading Times
2.2. Transcriptome Sequencing and Analysis of DEGs in Soybean
2.3. KEGG Enrichment Analysis of DEGs
2.4. Identification of Differentially Expressed Transcription Factors
2.5. Identification of Genes Involved in Photosynthesis
2.6. Weighted Gene Co-Expression Network Analysis (WGCNA)
2.7. Metabolomic Analysis
2.8. Combined Transcriptomic and Metabolomic Analyses
2.9. Screening and qRT-PCR Validation of Candidate Shattering Genes
3. Discussion
4. Materials and Methods
4.1. Plants and Sample Preparation
4.2. Physicochemical Properties
4.3. RNA-Seq Analysis
4.4. Weighted Gene Co-Expression Network Analysis (WGCNA)
4.5. Metabolite Profiling Analysis
4.6. Validation of Differentially Expressed Genes by qRT-PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jiang, A.; Liu, J.; Gao, W.; Ma, R.; Zhang, J.; Zhang, X.; Du, C.; Yi, Z.; Fang, X.; Zhang, J. Transcriptomic and Metabolomic Analyses Reveal the Key Genes Related to Shade Tolerance in Soybean. Int. J. Mol. Sci. 2023, 24, 14230. https://doi.org/10.3390/ijms241814230
Jiang A, Liu J, Gao W, Ma R, Zhang J, Zhang X, Du C, Yi Z, Fang X, Zhang J. Transcriptomic and Metabolomic Analyses Reveal the Key Genes Related to Shade Tolerance in Soybean. International Journal of Molecular Sciences. 2023; 24(18):14230. https://doi.org/10.3390/ijms241814230
Chicago/Turabian StyleJiang, Aohua, Jiaqi Liu, Weiran Gao, Ronghan Ma, Jijun Zhang, Xiaochun Zhang, Chengzhang Du, Zelin Yi, Xiaomei Fang, and Jian Zhang. 2023. "Transcriptomic and Metabolomic Analyses Reveal the Key Genes Related to Shade Tolerance in Soybean" International Journal of Molecular Sciences 24, no. 18: 14230. https://doi.org/10.3390/ijms241814230