The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients
Abstract
:1. Introduction
2. Results
2.1. LC/MS Analysis of Protein Profiles of Serum Exosomes of NSCLC Patients vs. Controls
2.2. Analysis of Hierarchical Clustering
2.3. Analysis of Protein–Protein Interactions in Serum Exosome-Derived Proteins
2.4. Analysis of Serum Exosome-Derived Protein Hub Clusters
2.5. Analysis of the Impact of NSCLC Patients Serum Exosome-Derived Proteins on Immune Cell Infiltration
2.6. Protein Profiles of Serum Exosomes of NSCLC Patients According to the Histopathological Subtype of Tumor
2.7. Protein Profiles of Serum Exosomes of NSCLC Patients According to the Tumor Size
2.8. Protein Profiles of Serum Exosomes of NSCLC Patients According to the State of Regional Lymph Nodes
3. Discussion
4. Materials and Methods
4.1. Patient Characteristics
4.2. Serum Collection
4.3. Isolation of Proteins from Serum Exosomes
4.4. LC/MS Analysis of Exosomal Proteins
4.5. Hierarchical Clustering
4.6. Protein–Protein Interaction Network
4.7. Analysis of Cancer-Associated Fibroblasts (CAFs) and M2 Macrophages Infiltration
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tumor Features | Number of Cases (n) | Total Percentage of Cases |
---|---|---|
Histopathological subtype of NSCLC | ||
SCC a | 6 | 40% |
AC b | 8 | 53% |
LCC c | 1 | 7% |
Stage of cancer development | ||
according to AJCC d staging system | ||
Stage I | 3 | 20% |
Stage II | 6 | 40% |
Stage III | 6 | 40% |
Presence of lymph node metastasis | ||
according to the pTNM e staging system | ||
N0 | 5 | 33% |
N1 | 6 | 40% |
N2 | 4 | 27% |
Tumor size according to the pTNM staging system | ||
T1a + T1b | 6 | 40% |
T2a + T2b | 5 | 33% |
T3 + T4 | 4 | 27% |
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Baran, K.; Waśko, J.; Kryczka, J.; Boncela, J.; Jabłoński, S.; Kolesińska, B.; Brzeziańska-Lasota, E.; Kordiak, J. The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients. Int. J. Mol. Sci. 2023, 24, 13669. https://doi.org/10.3390/ijms241813669
Baran K, Waśko J, Kryczka J, Boncela J, Jabłoński S, Kolesińska B, Brzeziańska-Lasota E, Kordiak J. The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients. International Journal of Molecular Sciences. 2023; 24(18):13669. https://doi.org/10.3390/ijms241813669
Chicago/Turabian StyleBaran, Kamila, Joanna Waśko, Jakub Kryczka, Joanna Boncela, Sławomir Jabłoński, Beata Kolesińska, Ewa Brzeziańska-Lasota, and Jacek Kordiak. 2023. "The Comparison of Serum Exosome Protein Profile in Diagnosis of NSCLC Patients" International Journal of Molecular Sciences 24, no. 18: 13669. https://doi.org/10.3390/ijms241813669