Integration of Single-Cell RNA Sequencing and Bulk RNA Sequencing Reveals That TAM2-Driven Genes Affect Immunotherapeutic Response and Prognosis in Pancreatic Cancer
Abstract
:1. Introduction
2. Results
2.1. Identification of TAM2 Co-Expressed Genes in PC
2.2. Screening for TAM2-Driven Genes at the Single-Cell Level
2.3. Molecular Typing Based on TAM2-Driven Genes
2.4. Immune Infiltration Analysis and Immunotherapy Prediction
2.5. Targeted Drugs Sensitivity Analysis and Mutation Profiling
2.6. Biological Mechanisms of Different Clusters
2.7. Construction of a Prognostic Model Based on the TAM2-Driven Genes
2.8. Assessing the Performance of a Prognostic Model
2.9. Confirmation of Critical TAM2-Driven Genes
3. Discussion
4. Materials and Methods
4.1. Data Acquisition
4.2. Immune Cell Infiltration Analysis
4.3. Weighted Correlation Network Analysis (WGCNA)
4.4. Single-Cell Sequencing Data Download and Processing
4.5. Consensus Clustering
4.6. Targeted Drug Sensitivity Analysis and Immunotherapy Prediction
4.7. Functional Enrichment Analysis of Different Clusters
4.8. COX Risk Model Construction
4.9. Cell Culture and Treatments
4.10. RNA Extraction and qRT-PCR
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Du, Y.; Dong, S.; Jiang, W.; Li, M.; Li, W.; Li, X.; Zhou, W. Integration of Single-Cell RNA Sequencing and Bulk RNA Sequencing Reveals That TAM2-Driven Genes Affect Immunotherapeutic Response and Prognosis in Pancreatic Cancer. Int. J. Mol. Sci. 2023, 24, 12787. https://doi.org/10.3390/ijms241612787
Du Y, Dong S, Jiang W, Li M, Li W, Li X, Zhou W. Integration of Single-Cell RNA Sequencing and Bulk RNA Sequencing Reveals That TAM2-Driven Genes Affect Immunotherapeutic Response and Prognosis in Pancreatic Cancer. International Journal of Molecular Sciences. 2023; 24(16):12787. https://doi.org/10.3390/ijms241612787
Chicago/Turabian StyleDu, Yan, Shi Dong, Wenkai Jiang, Mengyao Li, Wancheng Li, Xin Li, and Wence Zhou. 2023. "Integration of Single-Cell RNA Sequencing and Bulk RNA Sequencing Reveals That TAM2-Driven Genes Affect Immunotherapeutic Response and Prognosis in Pancreatic Cancer" International Journal of Molecular Sciences 24, no. 16: 12787. https://doi.org/10.3390/ijms241612787