Co-Existence of blaNDM-1, blaOXA-23, blaOXA-64, blaPER-7 and blaADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt

Sánchez-Urtaza, Sandra; Ocampo-Sosa, Alain; Molins-Bengoetxea, Ainhoa; Rodríguez-Grande, Jorge; El-Kholy, Mohammed A.; Hernandez, Marta; Abad, David; Shawky, Sherine M.; Alkorta, Itziar; Gallego, Lucia

doi:10.3390/ijms241512515

Open AccessCommunication

Co-Existence of bla_NDM-1, bla_OXA-23, bla_OXA-64, bla_PER-7 and bla_ADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt

by

Sandra Sánchez-Urtaza

¹

,

Alain Ocampo-Sosa

^2,3,*,

Ainhoa Molins-Bengoetxea

¹,

Jorge Rodríguez-Grande

²

,

Mohammed A. El-Kholy

⁴

,

Marta Hernandez

⁵

,

David Abad

⁵

,

Sherine M. Shawky

⁶,

Itziar Alkorta

⁷

and

Lucia Gallego

^1,*

¹

Department of Immunology, Microbiology and Parasitology, Faculty of Medicine and Nursing, University of the Basque Country, 48940 Leioa, Spain

²

Microbiology Service, Health Research Institute (IDIVAL), University Hospital Marqués de Valdecilla, 39008 Santander, Spain

³

CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain

⁴

Division of Clinical and Biological Sciences, Department of Microbiology and Biotechnology, College of Pharmacy, Arab Academy for Science, Technology & Maritime Transport (AASTMT), Alexandria P.O. Box 1029, Egypt

⁵

Laboratory of Molecular Biology and Microbiology, One Health, Agrarian Technological Institute of Castile and Leon (ITACyL), 47009 Valladolid, Spain

⁶

Medical Research Institute, Alexandria University, Alexandria 5422031, Egypt

⁷

Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country, 48940 Leioa, Spain

^*

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2023, 24(15), 12515; https://doi.org/10.3390/ijms241512515

Submission received: 3 July 2023 / Revised: 30 July 2023 / Accepted: 5 August 2023 / Published: 7 August 2023

(This article belongs to the Special Issue Antimicrobial Resistance, Molecular Epidemiology and Emerging Therapeutic Strategies in Carbapenem-Resistant Gram-Negative Bacteria)

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Abstract

The increasing rates of antimicrobial resistance among carbapenem-resistant Acinetobacter baumannii in the Middle East and North Africa are one of the major concerns for healthcare settings. We characterised the first A. baumannii isolate harbouring five β-lactamases identified in Egypt. The isolate Ale25 was obtained from an ICU patient of a hospital from Alexandria. The isolate was phenotypically and genotypically screened for carbapenemase genes. The isolate was resistant to carbapenems, aminoglycosides, fluoroquinolones and cefiderocol. Whole-Genome Sequencing identified five β-lactamase genes, bla_NDM-1, bla_OXA-23, bla_OXA-64, bla_PER-7 and bla_ADC-57, together with other antibiotic resistance genes, conferring resistance to sulfonamides, macrolides, tetracyclines, rifamycin and chloramphenicol. Virulome analysis showed the presence of genes involved in adhesion and biofilm production, type II and VI secretion systems, exotoxins, etc. Multi-Locus Sequence Typing analysis identified the isolate as Sequence Types 113^Pas and 2246^Oxf, belonging to International Clone 7. Sequencing experiments revealed the presence of four plasmids of 2.7, 22.3, 70.4 and 240.8 Kb. All the β-lactamase genes were located in the chromosome, except the bla_PER-7, gene which was found within the plasmid of 240.8 Kb. This study highlights the threat of the emergence and dissemination of these types of isolates.

Keywords: Acinetobacter baumannii; carbapenemase genes; whole genome sequencing

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MDPI and ACS Style

Sánchez-Urtaza, S.; Ocampo-Sosa, A.; Molins-Bengoetxea, A.; Rodríguez-Grande, J.; El-Kholy, M.A.; Hernandez, M.; Abad, D.; Shawky, S.M.; Alkorta, I.; Gallego, L. Co-Existence of bla_NDM-1, bla_OXA-23, bla_OXA-64, bla_PER-7 and bla_ADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt. Int. J. Mol. Sci. 2023, 24, 12515. https://doi.org/10.3390/ijms241512515

AMA Style

Sánchez-Urtaza S, Ocampo-Sosa A, Molins-Bengoetxea A, Rodríguez-Grande J, El-Kholy MA, Hernandez M, Abad D, Shawky SM, Alkorta I, Gallego L. Co-Existence of bla_NDM-1, bla_OXA-23, bla_OXA-64, bla_PER-7 and bla_ADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt. International Journal of Molecular Sciences. 2023; 24(15):12515. https://doi.org/10.3390/ijms241512515

Chicago/Turabian Style

Sánchez-Urtaza, Sandra, Alain Ocampo-Sosa, Ainhoa Molins-Bengoetxea, Jorge Rodríguez-Grande, Mohammed A. El-Kholy, Marta Hernandez, David Abad, Sherine M. Shawky, Itziar Alkorta, and Lucia Gallego. 2023. "Co-Existence of bla_NDM-1, bla_OXA-23, bla_OXA-64, bla_PER-7 and bla_ADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt" International Journal of Molecular Sciences 24, no. 15: 12515. https://doi.org/10.3390/ijms241512515

APA Style

Sánchez-Urtaza, S., Ocampo-Sosa, A., Molins-Bengoetxea, A., Rodríguez-Grande, J., El-Kholy, M. A., Hernandez, M., Abad, D., Shawky, S. M., Alkorta, I., & Gallego, L. (2023). Co-Existence of bla_NDM-1, bla_OXA-23, bla_OXA-64, bla_PER-7 and bla_ADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt. International Journal of Molecular Sciences, 24(15), 12515. https://doi.org/10.3390/ijms241512515

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Co-Existence of bla_NDM-1, bla_OXA-23, bla_OXA-64, bla_PER-7 and bla_ADC-57 in a Clinical Isolate of Acinetobacter baumannii from Alexandria, Egypt

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