GTS-21 Enhances Regulatory T Cell Development from T Cell Receptor-Activated Human CD4+ T Cells Exhibiting Varied Levels of CHRNA7 and CHRFAM7A Expression
Abstract
1. Introduction
2. Results
2.1. mRNA Expression of α7 and Dupα7 Subunits
2.1.1. Under the Resting Conditions
2.1.2. Changes during TCR-Activation
2.2. Effects of GTS-21 on CD4+ T Cell Proliferation and Treg Development
2.2.1. Proliferation
2.2.2. Treg Development
3. Discussion
3.1. Expression of mRNAs for α7 and Dupα7 Subunits in Resting Human CD4+ T Cells
3.2. Expression of α7 and Dupα7 Subunit mRNAs during TCR Activation
3.3. Effects of GTS-21 on Proliferation
3.4. The Effect of GTS-21 on Treg Development in TCR-Activated CD4+ T Cells
4. Materials and Methods
4.1. Cell Culture
4.1.1. CHRNA7 and CHRFAM7A Expression under the Resting Conditions
4.1.2. CHRNA7 and CHRFAM7A Expression during T Cell Activation
4.1.3. Effects of GTS-21 on TCR-Activated T Cell Proliferation
4.1.4. Effects of GTS-21 on Treg Development in TCR-Activated T Cells
4.2. Real-Time PCR
4.3. Flow Cytometry for Treg Development
4.4. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mashimo, M.; Fujii, T.; Ono, S.; Moriwaki, Y.; Misawa, H.; Azami, T.; Kasahara, T.; Kawashima, K. GTS-21 Enhances Regulatory T Cell Development from T Cell Receptor-Activated Human CD4+ T Cells Exhibiting Varied Levels of CHRNA7 and CHRFAM7A Expression. Int. J. Mol. Sci. 2023, 24, 12257. https://doi.org/10.3390/ijms241512257
Mashimo M, Fujii T, Ono S, Moriwaki Y, Misawa H, Azami T, Kasahara T, Kawashima K. GTS-21 Enhances Regulatory T Cell Development from T Cell Receptor-Activated Human CD4+ T Cells Exhibiting Varied Levels of CHRNA7 and CHRFAM7A Expression. International Journal of Molecular Sciences. 2023; 24(15):12257. https://doi.org/10.3390/ijms241512257
Chicago/Turabian StyleMashimo, Masato, Takeshi Fujii, Shiro Ono, Yasuhiro Moriwaki, Hidemi Misawa, Tetsushi Azami, Tadashi Kasahara, and Koichiro Kawashima. 2023. "GTS-21 Enhances Regulatory T Cell Development from T Cell Receptor-Activated Human CD4+ T Cells Exhibiting Varied Levels of CHRNA7 and CHRFAM7A Expression" International Journal of Molecular Sciences 24, no. 15: 12257. https://doi.org/10.3390/ijms241512257
APA StyleMashimo, M., Fujii, T., Ono, S., Moriwaki, Y., Misawa, H., Azami, T., Kasahara, T., & Kawashima, K. (2023). GTS-21 Enhances Regulatory T Cell Development from T Cell Receptor-Activated Human CD4+ T Cells Exhibiting Varied Levels of CHRNA7 and CHRFAM7A Expression. International Journal of Molecular Sciences, 24(15), 12257. https://doi.org/10.3390/ijms241512257