Short- and Long-Term Regulation of HuD: A Molecular Switch Mediated by Folic Acid?
Abstract
:1. Introduction
2. Results
2.1. Protein–Protein Interaction Network of the Targets Controlled by HuD
2.2. Effect of Folic Acid on Cell Viability and BDNF mRNA Content
2.3. Effect of Folic Acid on HuD and BDNF Expression
2.4. HuD Post-Translational Modification after Prolonged Folic Acid Treatment
3. Discussion
4. Material and Methods
4.1. Protein–Protein Interaction (PPI) Network
4.2. Cell Culture
4.3. MTT Assay
4.4. Western Blotting
4.5. Real-Time Quantitative RT-PCR
4.6. Immunoprecipitation
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Marchesi, N.; Linciano, P.; Campagnoli, L.I.M.; Fahmideh, F.; Rossi, D.; Costa, G.; Ambrosio, F.A.; Barbieri, A.; Collina, S.; Pascale, A. Short- and Long-Term Regulation of HuD: A Molecular Switch Mediated by Folic Acid? Int. J. Mol. Sci. 2023, 24, 12201. https://doi.org/10.3390/ijms241512201
Marchesi N, Linciano P, Campagnoli LIM, Fahmideh F, Rossi D, Costa G, Ambrosio FA, Barbieri A, Collina S, Pascale A. Short- and Long-Term Regulation of HuD: A Molecular Switch Mediated by Folic Acid? International Journal of Molecular Sciences. 2023; 24(15):12201. https://doi.org/10.3390/ijms241512201
Chicago/Turabian StyleMarchesi, Nicoletta, Pasquale Linciano, Lucrezia Irene Maria Campagnoli, Foroogh Fahmideh, Daniela Rossi, Giosuè Costa, Francesca Alessandra Ambrosio, Annalisa Barbieri, Simona Collina, and Alessia Pascale. 2023. "Short- and Long-Term Regulation of HuD: A Molecular Switch Mediated by Folic Acid?" International Journal of Molecular Sciences 24, no. 15: 12201. https://doi.org/10.3390/ijms241512201