APOA2: New Target for Molecular Hydrogen Therapy in Sepsis-Related Lung Injury Based on Proteomic and Genomic Analysis
Abstract
:1. Introduction
2. Results
2.1. Proteomics Target Proteins Analysis
2.2. Pathway Analysis and GO Analysis
2.3. Differential Expressional Genes Analysis
2.4. PPI Network Construction
2.5. Common Hydrogen Treatment Protein Screening
2.6. Results of SMR and Drug-Target MR
2.7. Syndrome–Target and pQTL MR Analysis
2.8. Results of ELISA and IF
3. Discussion
4. Materials and Methods
4.1. Animal Model Construction
4.2. Proteomics Collection and Processing
4.3. Transcriptomic Collection and Processing
4.4. Data Pre-Processing and Differential Analysis for Proteomics
4.5. Data Pre-Processing for Transcriptome
4.6. Differentially Expressional Genes’ Screening
4.7. Protein–Protein Interaction Network and Hub Biomarkers’ Identification
4.8. SMR Analysis
4.9. Two Sample and Drug–Target Mendelian Randomizations for Target mRNA
4.10. pQTL Analysis for Target Proteins
4.11. Enzyme Linked Immunosorbent Assay
4.12. Immunofluorescence of Lung
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cochran Q-Test of IVW | Egger Intercept | MR-PRESSO | |
---|---|---|---|
HDL and sepsis | Q = 74.7 p-value = 0.13 | Egger intercept = 0.003 p-value = 0.48 | Global test = 77.2 p-value = 0.16 |
Type 2 diabetes and sepsis | Q = 68.9 p-value = 0.38 | Egger intercept = −0.004 p-value = 0.51 | Global test = 71.5 p-value = 0.43 |
Apoa 2 and pneumonia | Q = 7.04 p-value = 0.13 | Egger intercept = −0.01 p-value = 0.51 | Global test = 12.09 p-value = 0.33 |
Apoa 2 and heart failure | Q = 4.39 p-value = 0.22 | Egger intercept = −0.01 p-value = 0.67 | Global test = 7.13 p-value = 0.45 |
pQTLs | Q = 1.93 p-value = 0.59 | Egger intercept = −0.02 p-value = 0.58 | Global test = 2.73 p-value = 0.96 |
β_GWAS | P_GWAS | β_eQTL | P_eQTL | β_SMR | P_SMR | |
---|---|---|---|---|---|---|
Apoa2 | −0.0375 | 0.008 | 0.143 | 5.26 × 10−62 | −0.263 | 0.011 |
Agonists | −0.77 | 0.03 | ||||
Antagonists | 0.11 | 0.02 |
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Wang, Y.; Fan, Y.; Jiang, Y.; Wang, E.; Song, Y.; Chen, H.; Xu, F.; Xie, K.; Yu, Y. APOA2: New Target for Molecular Hydrogen Therapy in Sepsis-Related Lung Injury Based on Proteomic and Genomic Analysis. Int. J. Mol. Sci. 2023, 24, 11325. https://doi.org/10.3390/ijms241411325
Wang Y, Fan Y, Jiang Y, Wang E, Song Y, Chen H, Xu F, Xie K, Yu Y. APOA2: New Target for Molecular Hydrogen Therapy in Sepsis-Related Lung Injury Based on Proteomic and Genomic Analysis. International Journal of Molecular Sciences. 2023; 24(14):11325. https://doi.org/10.3390/ijms241411325
Chicago/Turabian StyleWang, Yuanlin, Yan Fan, Yi Jiang, Enquan Wang, Yu Song, Hongguang Chen, Feier Xu, Keliang Xie, and Yonghao Yu. 2023. "APOA2: New Target for Molecular Hydrogen Therapy in Sepsis-Related Lung Injury Based on Proteomic and Genomic Analysis" International Journal of Molecular Sciences 24, no. 14: 11325. https://doi.org/10.3390/ijms241411325