Probing Transcriptional Crosstalk between Cryptochromes and Iron-sulfur Cluster Assembly 1 (MagR) in the Magnetoresponse of a Migratory Insect
Abstract
:1. Introduction
2. Results
2.1. Transcriptional Responses of the Putative Magnetosensing Genes to Magnetic Field Intensity Changes
2.2. Transcript Expression of Cryptochromes after Knocking down the Putative Magnetoreceptor Iron-sulfur Cluster Assembly 1 (MagR)
2.3. Transcript Expression of the Putative Magnetoreceptor Iron-sulfur Cluster Assembly 1 after Knocking down Cryptochrome 1 or Cryptochrome 2
2.4. Potential Transcriptional Interactions between Cryptochrome 1 and Cryptochrome 2
2.5. Transcript Expression of the Putative Magnetoreceptor Iron-sulfur Cluster Assembly 1 after Knocking down Both Cryptochrome 1 and Cryptochrome 2
3. Discussion
4. Materials and Methods
4.1. Insects
4.2. Exposure of Insects to Magnetic Fields
4.3. RNA Isolation and cDNA Synthesis
4.4. Double-Stranded RNA Preparation
4.5. Microinjection of dsRNA
4.6. Transcript Expression Analysis
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, Y.; Zhang, Y.; Zhao, J.; He, J.; Xuanyuan, Z.; Pan, W.; Sword, G.A.; Chen, F.; Wan, G. Probing Transcriptional Crosstalk between Cryptochromes and Iron-sulfur Cluster Assembly 1 (MagR) in the Magnetoresponse of a Migratory Insect. Int. J. Mol. Sci. 2023, 24, 11101. https://doi.org/10.3390/ijms241311101
Zhang Y, Zhang Y, Zhao J, He J, Xuanyuan Z, Pan W, Sword GA, Chen F, Wan G. Probing Transcriptional Crosstalk between Cryptochromes and Iron-sulfur Cluster Assembly 1 (MagR) in the Magnetoresponse of a Migratory Insect. International Journal of Molecular Sciences. 2023; 24(13):11101. https://doi.org/10.3390/ijms241311101
Chicago/Turabian StyleZhang, Yuning, Ying Zhang, Jingyu Zhao, Jinglan He, Zongjin Xuanyuan, Weidong Pan, Gregory A. Sword, Fajun Chen, and Guijun Wan. 2023. "Probing Transcriptional Crosstalk between Cryptochromes and Iron-sulfur Cluster Assembly 1 (MagR) in the Magnetoresponse of a Migratory Insect" International Journal of Molecular Sciences 24, no. 13: 11101. https://doi.org/10.3390/ijms241311101