Comparative Study of Nanoparticle Blood Circulation after Forced Clearance of Own Erythrocytes (Mononuclear Phagocyte System-Cytoblockade) or Administration of Cytotoxic Doxorubicin- or Clodronate-Loaded Liposomes
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of Liposomes
2.2. Synthesis and Characterization of MNPs
2.3. Investigation of the Prolongation of MNP Circulation in the Bloodstream
2.4. Biodistribution Studies
3. Materials and Methods
3.1. Materials
3.2. Animals
3.3. Liposome Preparation and Loading with Doxorubicin (Dox)
3.4. Liposome Preparation and Loading with Clodronic Acid (CA)
3.5. Characterization of Liposomes
3.6. Synthesis of Superparamagnetic Iron Oxide Nanoparticles (SPIONs)
3.7. Characterization of Magnetic Nanoparticles (MNPs)
3.8. Measurement of Magnetic Nanoparticle Circulation Half-Life in the Bloodstream of Mice
3.9. Labeling of Liposomes
3.10. Labeling of 200 nm Estapor Magnetic Nanoparticles
3.11. Biodistribution of Magnetic Nanoparticles and Liposomes
3.12. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Nanoparticles | Manufacturer | Mean Hydrodynamic Diameter ± SD, nm | Zeta Potential ± SD, mV |
---|---|---|---|
Carboxymethyl dextran-coated SPIONs | This work | 97.5 ± 2.9 | −18.3 ± 1.0 |
fluidMAG-CMX carboxymethyl dextran-coated magnetic beads | Chemicell, Germany | 171.2 ± 18.0 | 6.9 ± 0.2 |
Estapor carboxylated polystyrene magnetic beads | Merck Millipore, USA | 190.6 ± 1.7 | −12.5 ± 1.2 |
Estapor tosyl-activated polystyrene magnetic beads | Merck Millipore, USA | 957.2 ± 150.2 | 15.1 ± 0.6 |
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Mochalova, E.N.; Egorova, E.A.; Komarova, K.S.; Shipunova, V.O.; Khabibullina, N.F.; Nikitin, P.I.; Nikitin, M.P. Comparative Study of Nanoparticle Blood Circulation after Forced Clearance of Own Erythrocytes (Mononuclear Phagocyte System-Cytoblockade) or Administration of Cytotoxic Doxorubicin- or Clodronate-Loaded Liposomes. Int. J. Mol. Sci. 2023, 24, 10623. https://doi.org/10.3390/ijms241310623
Mochalova EN, Egorova EA, Komarova KS, Shipunova VO, Khabibullina NF, Nikitin PI, Nikitin MP. Comparative Study of Nanoparticle Blood Circulation after Forced Clearance of Own Erythrocytes (Mononuclear Phagocyte System-Cytoblockade) or Administration of Cytotoxic Doxorubicin- or Clodronate-Loaded Liposomes. International Journal of Molecular Sciences. 2023; 24(13):10623. https://doi.org/10.3390/ijms241310623
Chicago/Turabian StyleMochalova, Elizaveta N., Elena A. Egorova, Kristina S. Komarova, Victoria O. Shipunova, Nelli F. Khabibullina, Petr I. Nikitin, and Maxim P. Nikitin. 2023. "Comparative Study of Nanoparticle Blood Circulation after Forced Clearance of Own Erythrocytes (Mononuclear Phagocyte System-Cytoblockade) or Administration of Cytotoxic Doxorubicin- or Clodronate-Loaded Liposomes" International Journal of Molecular Sciences 24, no. 13: 10623. https://doi.org/10.3390/ijms241310623