Circulating miRNAs Expression in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Abstract
:1. Introduction
2. Results
2.1. Epidemiological and Clinical Features of ME/CFS and CTR Groups
2.2. Quantification of Pro-Inflammatory Cytokines in Plasma Samples
2.3. HHV-6A/B Presence and Load
2.4. miRNA Plasma Levels in ME/CFS Patients
2.5. Gene Pathways Analysis
3. Discussion
4. Materials and Methods
4.1. Study Population
4.2. Samples Collection
4.3. Cytokines Evaluation
4.4. DNA Extraction and Analyses of HHV-6A/B Presence
4.5. RNA Extraction and miRNA Analysis
4.6. Gene Pathways and Functional Enriched Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Target miRNAs | Association and Potential Role in ME/CFS | References |
---|---|---|
miR-124-3p |
| |
miR-127-3p |
| |
miR-140-5p |
| |
miR-142-5p |
| |
miR-143-3p |
| |
miR-150-5p |
| |
miR-448 |
| |
miR-551b-3p |
|
Group | N | Age Mean ± SE 1 | Gender | ME/CFS Severity 2 | ||
---|---|---|---|---|---|---|
1 | 2 | 3 | ||||
ME/CFS | 40 | 49.30 ± 2.234 | F: 31 (77.5%) M: 9 (22.5%) | 5 (12.5%) | 22 (55%) | 13 (32.5%) |
Controls | 20 | 33.40 ± 2.634 | F: 16 (80%) M: 4 (40%) | - | - | - |
p value | 0.0001 | 1.00 (n.s) |
Cytokine | Group (Subjects n°) | ||||||||
---|---|---|---|---|---|---|---|---|---|
Controls (20) | ME/CFS (39) | p-Value 2 | ME/CFS Grade 1 (5) | p-Value 2 | ME/CFS Grade 2 (22) | p-Value 2 | ME/CFS Grade 3 (13) | p-Value 2 | |
IFN-γ | 649.90 ± 61.33 | 507.7 ± 49.98 | 0.1560 | 675.8 ± 92.42 | 0.2890 | 429.2 ± 77.06 | 0.1634 | 572.3 ± 79.80 | 0.5089 |
IL-17A | 103.80 ± 12.40 | 18.80 ± 5.54 | 0.0003 | 20.47 ± 20.43 | 0.1568 | 16.66 ± 5.55 | <0.0001 | 19.48 ± 11.76 | 0.0043 |
IL-2 | 30.40 ± 3.99 | 4.52 ± 1.42 | 0.0001 | 7.26 ± 7.17 | 0.1989 | 4.28 ± 1.10 | <0.0001 | 4.40 ± 2.63 | 0.0004 |
IL-21 | 43.97 ± 5.94 | 9.07 ± 2.13 | 0.0001 | 10.46 ± 9.20 | 0.1065 | 8.68 ± 2.18 | <0.0001 | 9.64 ± 3.99 | 0.0005 |
IL-23 | 1.602 ± 235.00 | 407.5 ± 114.5 | 0.1065 | 527.5 ± 620.3 | 0.6728 | 403.2 ± 100.3 | 0.0013 | 377.4 ± 194.3 | 0.0156 |
IL-6 | 16.34 ± 8.14 | 0.90 ± 2.13 | 0.0270 | 0.95 ± 0.23 | 0.0061 | 0.90 ± 2.96 | 0.0308 | 0.74 ± 4.31 | 0.0588 |
TNF-α | 45.16 ± 4.06 | 12.35 ± 3.42 | 0.0052 | 13.44 ± 11.27 | 0.6397 | 12.35 ±4.04 | 0.0038 | 11.61 ± 6.89 | 0.0587 |
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Soffritti, I.; Gravelsina, S.; D’Accolti, M.; Bini, F.; Mazziga, E.; Vilmane, A.; Rasa-Dzelzkaleja, S.; Nora-Krukle, Z.; Krumina, A.; Murovska, M.; et al. Circulating miRNAs Expression in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome. Int. J. Mol. Sci. 2023, 24, 10582. https://doi.org/10.3390/ijms241310582
Soffritti I, Gravelsina S, D’Accolti M, Bini F, Mazziga E, Vilmane A, Rasa-Dzelzkaleja S, Nora-Krukle Z, Krumina A, Murovska M, et al. Circulating miRNAs Expression in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome. International Journal of Molecular Sciences. 2023; 24(13):10582. https://doi.org/10.3390/ijms241310582
Chicago/Turabian StyleSoffritti, Irene, Sabine Gravelsina, Maria D’Accolti, Francesca Bini, Eleonora Mazziga, Anda Vilmane, Santa Rasa-Dzelzkaleja, Zaiga Nora-Krukle, Angelika Krumina, Modra Murovska, and et al. 2023. "Circulating miRNAs Expression in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome" International Journal of Molecular Sciences 24, no. 13: 10582. https://doi.org/10.3390/ijms241310582