C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis
Abstract
1. Introduction
2. Results
2.1. Meningitic E. coli Significantly Stimulates CXCL3 Expression In Vivo and In Vitro
2.2. Microglia Express CXCR2 during Meningitic E. coli Infection
2.3. CXCL3 Facilitated Microglia Polarization towards a Pro-Inflammatory Profile
2.4. CXCL3 Regulates Microglia Polarization via the Extracellular Signal-Regulated Protein Kinases 1 and 2 (ERK1/2) Pathway
2.5. CXCR2 Blockade Inhibits the Expression of Pro-Inflammatory Factors
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Cell Culture
4.2. Meningitic E. coli Infection of hBMECs and U251 Cells
4.3. Western Blotting
4.4. Isolation and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Analysis of RNA
4.5. Secretory CXCL3 Determination by Enzyme-Linked Immunosorbent Assay (ELISA)
4.6. Flow Cytometry Analysis
4.7. Immunofluorescence Analysis
4.8. Animal Infection Assay
4.9. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Qu, X.; Dou, B.; Yang, R.; Tan, C.; Chen, H.; Wang, X. C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis. Int. J. Mol. Sci. 2023, 24, 10432. https://doi.org/10.3390/ijms241310432
Qu X, Dou B, Yang R, Tan C, Chen H, Wang X. C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis. International Journal of Molecular Sciences. 2023; 24(13):10432. https://doi.org/10.3390/ijms241310432
Chicago/Turabian StyleQu, Xinyi, Beibei Dou, Ruicheng Yang, Chen Tan, Huanchun Chen, and Xiangru Wang. 2023. "C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis" International Journal of Molecular Sciences 24, no. 13: 10432. https://doi.org/10.3390/ijms241310432
APA StyleQu, X., Dou, B., Yang, R., Tan, C., Chen, H., & Wang, X. (2023). C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis. International Journal of Molecular Sciences, 24(13), 10432. https://doi.org/10.3390/ijms241310432