Characterizing the Spatiotemporal Transcriptomic Response of the Right Ventricle to Acute Pressure Overload
Abstract
:1. Introduction
2. Results
2.1. How Is the Initial (First 18 h) Transcriptomic Signature of the RV Impacted by the Timing and Severity of Acute Pressure Overload?
2.2. What Are the Pathways Associated with Acute RV Pressure Overload Induced by PE and How Do They Compare to Experimental Models of Pulmonary Hypertension (Sugen-Hypoxia and Monocrotaline)?
3. Discussion
3.1. The Relationship between Acute RV Pressure Overload Severity and Temporal Transcriptional Trajectory
3.2. Acute vs. Gradual Increase in RV Pressure Overload: Can PAB or PE Rodent Models Teach Us about RV Response in PH?
3.3. Spatiotemporal Transcriptomics at End-Stage Disease
4. Methods
4.1. Data Overview
4.2. Data Analysis, Normalization, and Pre-Processing
4.3. Dimensionality Reduction and Gene Set Enrichment Analysis
4.4. Statistical Methods
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Kheyfets, V.O.; Kumar, S.; Heerdt, P.M.; Ichimura, K.; Brown, R.D.; Lucero, M.; Essafri, I.; Williams, S.; Stenmark, K.R.; Spiekerkoetter, E. Characterizing the Spatiotemporal Transcriptomic Response of the Right Ventricle to Acute Pressure Overload. Int. J. Mol. Sci. 2023, 24, 9746. https://doi.org/10.3390/ijms24119746
Kheyfets VO, Kumar S, Heerdt PM, Ichimura K, Brown RD, Lucero M, Essafri I, Williams S, Stenmark KR, Spiekerkoetter E. Characterizing the Spatiotemporal Transcriptomic Response of the Right Ventricle to Acute Pressure Overload. International Journal of Molecular Sciences. 2023; 24(11):9746. https://doi.org/10.3390/ijms24119746
Chicago/Turabian StyleKheyfets, Vitaly O., Sushil Kumar, Paul M. Heerdt, Kenzo Ichimura, R. Dale Brown, Melissa Lucero, Ilham Essafri, Sarah Williams, Kurt R. Stenmark, and Edda Spiekerkoetter. 2023. "Characterizing the Spatiotemporal Transcriptomic Response of the Right Ventricle to Acute Pressure Overload" International Journal of Molecular Sciences 24, no. 11: 9746. https://doi.org/10.3390/ijms24119746