GmUFO1 Regulates Floral Organ Number and Shape in Soybean
Abstract
:1. Introduction
2. Results
2.1. Identification of UFO Genes
2.2. Spatial and Temporal Expression Profiles of the GmUFO1 Gene in Soybean Flower Development
2.3. CRISPR/Cas9 System Generated Target Mutations for GmUFO Genes
2.4. Knockout of the GmUFO1 Gene Caused Abnormal Flower Organs
2.5. Knockout of the GmUFO2 Gene Had No Effect on Flower Organs
2.6. Double Knockout of GmUFO1 and GmUFO2 Had More Serious Defects in Organ Number and Shape
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. GmUFO Identification and Cloning
4.3. Phylogenetic Analysis and Physical Properties of UFO Genes
4.4. In Situ Hybridization
4.5. Subcellular Localization
4.6. Cytological Analysis
4.7. Gene Knockout and Material Identification
4.8. Total RNA Extraction and qPCR
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yu, H.; Zhang, Y.; Fang, J.; Yang, X.; Zhang, Z.; Wang, F.; Wu, T.; Khan, M.H.U.; Bhat, J.A.; Jiang, Y.; et al. GmUFO1 Regulates Floral Organ Number and Shape in Soybean. Int. J. Mol. Sci. 2023, 24, 9662. https://doi.org/10.3390/ijms24119662
Yu H, Zhang Y, Fang J, Yang X, Zhang Z, Wang F, Wu T, Khan MHU, Bhat JA, Jiang Y, et al. GmUFO1 Regulates Floral Organ Number and Shape in Soybean. International Journal of Molecular Sciences. 2023; 24(11):9662. https://doi.org/10.3390/ijms24119662
Chicago/Turabian StyleYu, Huimin, Yaohua Zhang, Junling Fang, Xinjing Yang, Zhirui Zhang, Fawei Wang, Tao Wu, Muhammad Hafeez Ullah Khan, Javaid Akhter Bhat, Yu Jiang, and et al. 2023. "GmUFO1 Regulates Floral Organ Number and Shape in Soybean" International Journal of Molecular Sciences 24, no. 11: 9662. https://doi.org/10.3390/ijms24119662