Isoflavones Mediate Dendritogenesis Mainly through Estrogen Receptor α
Abstract
:1. Introduction
2. Results
2.1. Isoflavones Enhanced Cell Number and Dendrite Arborization in Purkinje Cells in the Primary Culture of Mouse Cerebellar Cells
2.2. GPER1 and ER Are Required for Isoflavone-Enhanced E2-Mediated Dendrite Arborization in Purkinje Cells
2.3. ERα Mediated Isoflavone-Induced Neurite Outgrowth in Neuro-2A Cells
2.4. Neuron–Glia Interaction Enhanced Isoflavone-Mediated Neuritogenesis
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Primary Culture of Mouse Cerebellar Cells
4.3. Primary Culture of Mouse Cerebellar Astrocytes
4.4. Mouse Neuro-2A and Co-Culture Studies
4.5. RNA Interference Assay
4.6. Western Blotting
4.7. Software and Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ariyani, W.; Amano, I.; Koibuchi, N. Isoflavones Mediate Dendritogenesis Mainly through Estrogen Receptor α. Int. J. Mol. Sci. 2023, 24, 9011. https://doi.org/10.3390/ijms24109011
Ariyani W, Amano I, Koibuchi N. Isoflavones Mediate Dendritogenesis Mainly through Estrogen Receptor α. International Journal of Molecular Sciences. 2023; 24(10):9011. https://doi.org/10.3390/ijms24109011
Chicago/Turabian StyleAriyani, Winda, Izuki Amano, and Noriyuki Koibuchi. 2023. "Isoflavones Mediate Dendritogenesis Mainly through Estrogen Receptor α" International Journal of Molecular Sciences 24, no. 10: 9011. https://doi.org/10.3390/ijms24109011