Long Non-Coding RNA GDAR Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes

Wang, Yong; Guo, Yunxia; Duan, Chunhui; Yang, Ruochen; Zhang, Lechao; Liu, Yueqin; Zhang, Yingjie

doi:10.3390/ijms23095183

Open AccessArticle

Long Non-Coding RNA GDAR Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes

by

Yong Wang

^1,†

,

Yunxia Guo

^2,†,

Chunhui Duan

¹,

Ruochen Yang

¹,

Lechao Zhang

¹,

Yueqin Liu

¹ and

Yingjie Zhang

^1,*

¹

College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China

²

College of Life Science, Hebei Agricultural University, Baoding 071000, China

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2022, 23(9), 5183; https://doi.org/10.3390/ijms23095183

Submission received: 11 April 2022 / Revised: 30 April 2022 / Accepted: 30 April 2022 / Published: 6 May 2022

(This article belongs to the Special Issue Noncoding RNAs: Molecular Mechanisms and Physio-Pathological Implications)

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Abstract

Short-term dietary supplementation of ewes during the luteal phase can increase fertility, most probably by stimulating glucose uptake by the follicles. However, the molecular mechanism of glucose regulation of follicular development has not yet been clarified, especially the further study of long non-coding RNA (lncRNA) in determining fertility during follicular development. We generated granulosa cell (GC) models of different doses of glucose (0, 2.1, 4.2, 8.4, 16.8 and 33.6 mM), and observed that the highest cell viability was recorded in the 8.4 mM group and the highest apoptosis rates were recorded in the 33.6 mM group. Therefore, a control group (n = 3, 0 mM glucose), a low glucose group (n = 3, add 8.4 mM glucose), and a high glucose group (n = 3, add 33.6 mM glucose) of GCs were created for next whole genomic RNA sequencing. In total, 18,172 novel lncRNAs and 510 annotated lncRNAs were identified in the GCs samples. Gene Ontology indicated that differentially expressed lncRNAs associated with cell apoptosis were highly enriched. Kyoto Encyclopedia of Genes and Genomes enrichment analysis of lncRNA target genes found that the apoptosis pathway and the p53 signaling pathway were both enriched. Furthermore, we focused on the function of a lncGDAR and verified that lncGDAR could influence cell apoptosis in GC development through affecting the mRNA and protein expression of apoptosis-related markers. These results provide the basis for further study of the lncRNA regulation mechanism in nutrition on female fertility.

Keywords: granulosa cell; glucose; apoptosis; long non-coding RNA

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MDPI and ACS Style

Wang, Y.; Guo, Y.; Duan, C.; Yang, R.; Zhang, L.; Liu, Y.; Zhang, Y. Long Non-Coding RNA GDAR Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes. Int. J. Mol. Sci. 2022, 23, 5183. https://doi.org/10.3390/ijms23095183

AMA Style

Wang Y, Guo Y, Duan C, Yang R, Zhang L, Liu Y, Zhang Y. Long Non-Coding RNA GDAR Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes. International Journal of Molecular Sciences. 2022; 23(9):5183. https://doi.org/10.3390/ijms23095183

Chicago/Turabian Style

Wang, Yong, Yunxia Guo, Chunhui Duan, Ruochen Yang, Lechao Zhang, Yueqin Liu, and Yingjie Zhang. 2022. "Long Non-Coding RNA GDAR Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes" International Journal of Molecular Sciences 23, no. 9: 5183. https://doi.org/10.3390/ijms23095183

APA Style

Wang, Y., Guo, Y., Duan, C., Yang, R., Zhang, L., Liu, Y., & Zhang, Y. (2022). Long Non-Coding RNA GDAR Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes. International Journal of Molecular Sciences, 23(9), 5183. https://doi.org/10.3390/ijms23095183

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Long Non-Coding RNA GDAR Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes

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