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Article

Actin Stress Fibers Response and Adaptation under Stretch

1
Cellular Mechanics Laboratory, Physics Department, SMAT-C, Universidad de Santiago de Chile, Santiago 9170124, Chile
2
Institut du Cerveau et de la Moelle Épinière, Hôpital Pitié Salpêtrière, 47 bd de l’Hôpital, 75013 Paris, France
3
Sorbonne Université, Faculté des Sciences et Ingénierie, UFR 926 Chemistry, 75005 Paris, France
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Institut Pierre Gilles de Gennes, Paris Sciences et Lettres Research University, 75005 Paris, France
5
Institut Curie, Paris Sciences et Lettres Research University, Centre National de la Recherche Scientifique, UMR 144, 75248 Paris, France
*
Authors to whom correspondence should be addressed.
Academic Editor: Miguel Vicente-Manzanares
Int. J. Mol. Sci. 2022, 23(9), 5095; https://doi.org/10.3390/ijms23095095
Received: 10 March 2022 / Revised: 11 April 2022 / Accepted: 14 April 2022 / Published: 3 May 2022
(This article belongs to the Special Issue Biophysics and Mechanical Properties of Cells)
One of the many effects of soft tissues under mechanical solicitation in the cellular damage produced by highly localized strain. Here, we study the response of peripheral stress fibers (SFs) to external stretch in mammalian cells, plated onto deformable micropatterned substrates. A local fluorescence analysis reveals that an adaptation response is observed at the vicinity of the focal adhesion sites (FAs) due to its mechanosensor function. The response depends on the type of mechanical stress, from a Maxwell-type material in compression to a complex scenario in extension, where a mechanotransduction and a self-healing process takes place in order to prevent the induced severing of the SF. A model is proposed to take into account the effect of the applied stretch on the mechanics of the SF, from which relevant parameters of the healing process are obtained. In contrast, the repair of the actin bundle occurs at the weak point of the SF and depends on the amount of applied strain. As a result, the SFs display strain-softening features due to the incorporation of new actin material into the bundle. In contrast, the response under compression shows a reorganization with a constant actin material suggesting a gliding process of the SFs by the myosin II motors. View Full-Text
Keywords: actin stress fibers; cell mechanics; SF response actin stress fibers; cell mechanics; SF response
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MDPI and ACS Style

Bernal, R.; Van Hemelryck, M.; Gurchenkov, B.; Cuvelier, D. Actin Stress Fibers Response and Adaptation under Stretch. Int. J. Mol. Sci. 2022, 23, 5095. https://doi.org/10.3390/ijms23095095

AMA Style

Bernal R, Van Hemelryck M, Gurchenkov B, Cuvelier D. Actin Stress Fibers Response and Adaptation under Stretch. International Journal of Molecular Sciences. 2022; 23(9):5095. https://doi.org/10.3390/ijms23095095

Chicago/Turabian Style

Bernal, Roberto, Milenka Van Hemelryck, Basile Gurchenkov, and Damien Cuvelier. 2022. "Actin Stress Fibers Response and Adaptation under Stretch" International Journal of Molecular Sciences 23, no. 9: 5095. https://doi.org/10.3390/ijms23095095

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