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Article

The Role of Cytoskeleton Revealed by Quartz Crystal Microbalance and Digital Holographic Microscopy

1
Department of Physics, University of Trieste, Via A. Valerio 2, 34127 Trieste, Italy
2
Consiglio Nazionale delle Ricerche (CNR), Istituto Officina dei Materiali (IOM), Area Science Park-Basovizza, Strada Statale 14, Km 163,5, 34149 Trieste, Italy
3
Department of Engineering and Architecture, University of Trieste, Via A. Valerio 6/A, 34127 Trieste, Italy
*
Author to whom correspondence should be addressed.
Present address: Department of Optical Nanoscopy, Max Planck Institute for Medical Research, Jahnstraße 29, 69120 Heidelberg, Germany.
Academic Editors: Miguel Vicente-Manzanares and Victor Muñoz
Int. J. Mol. Sci. 2022, 23(8), 4108; https://doi.org/10.3390/ijms23084108
Received: 8 March 2022 / Revised: 30 March 2022 / Accepted: 6 April 2022 / Published: 7 April 2022
(This article belongs to the Special Issue Biophysics and Mechanical Properties of Cells)
The connection between cytoskeleton alterations and diseases is well known and has stimulated research on cell mechanics, aiming to develop reliable biomarkers. In this study, we present results on rheological, adhesion, and morphological properties of primary rat cardiac fibroblasts, the cytoskeleton of which was altered by treatment with cytochalasin D (Cyt-D) and nocodazole (Noc), respectively. We used two complementary techniques: quartz crystal microbalance (QCM) and digital holographic microscopy (DHM). Qualitative data on cell viscoelasticity and adhesion changes at the cell–substrate near-interface layer were obtained with QCM, while DHM allowed the measurement of morphological changes due to the cytoskeletal alterations. A rapid effect of Cyt-D was observed, leading to a reduction in cell viscosity, loss of adhesion, and cell rounding, often followed by detachment from the surface. Noc treatment, instead, induced slower but continuous variations in the rheological behavior for four hours of treatment. The higher vibrational energy dissipation reflected the cell’s ability to maintain a stable attachment to the substrate, while a cytoskeletal rearrangement occurs. In fact, along with the complete disaggregation of microtubules at prolonged drug exposure, a compensatory effect of actin polymerization emerged, with increased stress fiber formation. View Full-Text
Keywords: QCM; DHM; cardiac fibroblasts; cytoskeleton; viscoelasticity; cell rheology QCM; DHM; cardiac fibroblasts; cytoskeleton; viscoelasticity; cell rheology
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MDPI and ACS Style

Braidotti, N.; do R. B. F. Lima, M.A.; Zanetti, M.; Rubert, A.; Ciubotaru, C.; Lazzarino, M.; Sbaizero, O.; Cojoc, D. The Role of Cytoskeleton Revealed by Quartz Crystal Microbalance and Digital Holographic Microscopy. Int. J. Mol. Sci. 2022, 23, 4108. https://doi.org/10.3390/ijms23084108

AMA Style

Braidotti N, do R. B. F. Lima MA, Zanetti M, Rubert A, Ciubotaru C, Lazzarino M, Sbaizero O, Cojoc D. The Role of Cytoskeleton Revealed by Quartz Crystal Microbalance and Digital Holographic Microscopy. International Journal of Molecular Sciences. 2022; 23(8):4108. https://doi.org/10.3390/ijms23084108

Chicago/Turabian Style

Braidotti, Nicoletta, Maria Augusta do R. B. F. Lima, Michele Zanetti, Alessandro Rubert, Catalin Ciubotaru, Marco Lazzarino, Orfeo Sbaizero, and Dan Cojoc. 2022. "The Role of Cytoskeleton Revealed by Quartz Crystal Microbalance and Digital Holographic Microscopy" International Journal of Molecular Sciences 23, no. 8: 4108. https://doi.org/10.3390/ijms23084108

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