Comparative Analysis of the Metabolome and Transcriptome between the Green and Yellow-Green Regions of Variegated Leaves in a Mutant Variety of the Tree Species Pteroceltis tatarinowii
Abstract
:1. Introduction
2. Results
2.1. Morphological Observations
2.2. Microstructure and Ultramicrostructure Observations
2.3. Determination of Pigment Contents
2.4. Assays of Protective Enzyme Activities and MDA Contents
2.5. RNA-Seq and Assembly Analyses
2.5.1. Quality Determination and Analysis of RNA Samples from Transcriptome Sequencing
2.5.2. Differentially Expressed Gene (DEG) Analysis
2.5.3. GO Classification and Enrichment Analysis of DEGs
2.5.4. KEGG Classification and Enrichment Analysis of DEGs
2.5.5. Flavonoid Biosynthesis Pathways in Different Leaf Colour Regions
2.5.6. Pigment- and Photosynthesis-Related Pathways
2.5.7. Amino Acid and Lipid Metabolic Pathways
2.5.8. Computer Prediction Analysis of Transcriptome Factors (TFs)
2.6. RT-qPCR Validation
2.7. Analysis of Differentially Accumulated Metabolites
2.7.1. Statistics and Analysis of Differentially Accumulated Metabolites
2.7.2. KEGG Annotations of Differentially Accumulated Metabolites
2.8. Correlation Analysis of DEGs and Differentially Accumulated Metabolites
3. Discussion
3.1. Explanation of Morphological Variation in P. tatarinowii ‘Jinyuyuan’ Leaves
3.2. Genes Associated with Variegated Leaf Colour Formation in P. tatarinowii ‘Jinyuyuan’
3.3. Analysis of the Redox Capacity of P. tatarinowii ‘Jinyuyuan’
4. Materials and Methods
4.1. Plant Materials
4.2. Morphological Observations
4.3. Microstructure and Ultramicrostructure Observations
4.4. Determination of Pigment Contents
4.5. Assays of Protective Enzyme Activities and MDA Contents
4.6. RNA Preparation, RNA Sequencing (RNA-seq) and Differential Gene Expression Analysis
4.7. RT-qPCR Validation
4.8. UPLC-MS
4.9. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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GR | YG | |
---|---|---|
L* | 47.70 ± 0.70 B | 56.70 ± 1.17 A |
a* | −7.68 ± 1.33 | −7.14 ± 0.73 |
b* | −5.46 ± 0.86 B | 4.80 ± 1.11 A |
c* | 9.50 ± 0.76 | 8.65 ± 0.82 |
h | 35.78 ± 8.45 A | −33.72 ± 6.72 B |
RHSCC | 137B | N144D |
Type | Upper Epidermis Thickness (μm) | Lower Epidermis Thickness (μm) | Leaf Thickness (μm) | Palisade Cells Thickness (μm) | Spongy Cells Thickness (μm) | Palisade Cells/Spongy Cells |
---|---|---|---|---|---|---|
GR | 8.580 ± 2.614 | 7.029 ± 1.668 | 105.585 ± 6.924 A | 44.626 ± 8.310 | 48.660 ± 7.197 a | 0.953 ± 0.316 |
YG | 8.487 ± 1.569 | 6.218 ± 1.509 | 84.288 ± 7.815 B | 37.644 ± 6.918 | 35.967 ± 7.809 b | 1.073 ± 0.219 |
GR | YG | |
---|---|---|
Chl a (mg g−1 FW) | 0.40 ± 0.0021 A | 0.31 ± 0.0003 B |
Chl b (mg g−1 FW) | 0.19 ± 0.0026 A | 0.15 ± 0.0024 B |
Chl a + b (mg g−1 FW) | 0.59 ± 0.0032 A | 0.47 ± 0.0028 B |
Chl a/b | 2.14 ± 0.0320 a | 2.06 ± 0.0307 b |
Car (ug g−1 FW) | 118.12 ± 0.0307 A | 110.50 ± 0.0178 B |
Chl a + b /Car | 4.97 ± 0.0269 A | 4.22 ± 0.0244 B |
Transcriptome | Metabolomic | ||||||
---|---|---|---|---|---|---|---|
Pathway ID | Description | Number | Pathway ID | Description | Number | ||
Down | Up | Down | Up | ||||
map00250 | Alanine, aspartate and glutamate metabolism | 2 | 0 | map00220 | Arginine biosynthesis | 3 | |
map00260 | Glycine, serine and threonine metabolism | 2 | 1 | map00250 | Alanine, aspartate and glutamate metabolism | 3 | |
map00270 | Cysteine and methionine metabolism | 4 | 0 | map00270 | Cysteine and methionine metabolism | 1 | |
map00280 | Valine, leucine and isoleucine degradation | 1 | 1 | map00330 | Arginine and proline metabolism | 2 | 1 |
map00300 | Lysine biosynthesis | 2 | 0 | map00340 | Histidine metabolism | 1 | |
map00310 | Lysine degradation | 1 | 0 | map00360 | Phenylalanine metabolism | 1 | |
map00330 | Arginine and proline metabolism | 5 | 0 | map00400 | Phenylalanine, tyrosine and tryptophan biosynthesis | 1 | |
map00340 | Histidine metabolism | 1 | 0 | map00430 | Taurine and hypotaurine metabolism | 1 | |
map00350 | Tyrosine metabolism | 4 | 1 | map00460 | Cyanoamino acid metabolism | 1 | |
map00360 | Phenylalanine metabolism | 4 | 1 | map00472 | D-Arginine and D-ornithine metabolism | 1 | |
map00380 | Tryptophan metabolism | 7 | 0 | map00480 | Glutathione metabolism | 2 | |
map00410 | beta-Alanine metabolism | 3 | 1 | ||||
map00430 | Taurine and hypotaurine metabolism | 2 | 0 | ||||
map00450 | Selenocompound metabolism | 1 | 0 | ||||
map00460 | Cyanoamino acid metabolism | 5 | 0 | ||||
map00480 | Glutathione metabolism | 5 | 1 |
Pathway ID | Description | Number | ||
---|---|---|---|---|
Down | Up | |||
Transcriptome | map00061 | Fatty acid biosynthesis | 1 | 1 |
map00062 | Fatty acid elongation | 2 | 0 | |
map00071 | Fatty acid degradation | 1 | ||
map00073 | Cutin, suberine and wax biosynthesis | 1 | ||
map00100 | Steroid biosynthesis | 1 | ||
map00561 | Glycerolipid metabolism | 6 | 0 | |
map00564 | Glycerophospholipid metabolism | 3 | ||
map00590 | Arachidonic acid metabolism | 1 | ||
map00591 | Linoleic acid metabolism | 2 | 0 | |
map00592 | alpha-Linolenic acid metabolism | 4 | 1 | |
map00600 | Sphingolipid metabolism | 2 | 0 | |
map01040 | Biosynthesis of unsaturated fatty acids | 1 | ||
Metabolomic | map00592 | alpha-Linolenic acid metabolism | 1 | 1 |
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Qiao, Q.; Wu, C.; Cheng, T.-T.; Yan, Y.; Zhang, L.; Wan, Y.-L.; Wang, J.-W.; Liu, Q.-Z.; Feng, Z.; Liu, Y. Comparative Analysis of the Metabolome and Transcriptome between the Green and Yellow-Green Regions of Variegated Leaves in a Mutant Variety of the Tree Species Pteroceltis tatarinowii. Int. J. Mol. Sci. 2022, 23, 4950. https://doi.org/10.3390/ijms23094950
Qiao Q, Wu C, Cheng T-T, Yan Y, Zhang L, Wan Y-L, Wang J-W, Liu Q-Z, Feng Z, Liu Y. Comparative Analysis of the Metabolome and Transcriptome between the Green and Yellow-Green Regions of Variegated Leaves in a Mutant Variety of the Tree Species Pteroceltis tatarinowii. International Journal of Molecular Sciences. 2022; 23(9):4950. https://doi.org/10.3390/ijms23094950
Chicago/Turabian StyleQiao, Qian, Chong Wu, Tian-Tian Cheng, Yu Yan, Lin Zhang, Ying-Lin Wan, Jia-Wei Wang, Qing-Zhong Liu, Zhen Feng, and Yan Liu. 2022. "Comparative Analysis of the Metabolome and Transcriptome between the Green and Yellow-Green Regions of Variegated Leaves in a Mutant Variety of the Tree Species Pteroceltis tatarinowii" International Journal of Molecular Sciences 23, no. 9: 4950. https://doi.org/10.3390/ijms23094950