A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry

Li, Fengxia; Fornasiero, Eugenio F.; Dankovich, Tal M.; Kluever, Verena; Rizzoli, Silvio O.

doi:10.3390/ijms23094615

Open AccessArticle

A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry

by

Fengxia Li

^1,2,*,

Eugenio F. Fornasiero

^1,2

,

Tal M. Dankovich

^1,3,

Verena Kluever

¹

and

Silvio O. Rizzoli

^1,2,*

¹

Center for Biostructural Imaging of Neurodegeneration, University Medical Center Göttingen, von-Siebold-Straße 3a, 37075 Göttingen, Germany

²

Department of Neuro- and Sensory Physiology, University Medical Center Göttingen, Humboldtallee 23, 37073 Göttingen, Germany

³

International Max Planck Research School for Neuroscience, Grisebachstr. 5, 37077 Göttingen, Germany

^*

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2022, 23(9), 4615; https://doi.org/10.3390/ijms23094615

Submission received: 3 March 2022 / Revised: 7 April 2022 / Accepted: 11 April 2022 / Published: 21 April 2022

(This article belongs to the Special Issue Nano-Materials and Methods 3.0)

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Abstract

Nano secondary ion mass spectrometry (nanoSIMS) imaging is a rapidly growing field in biological sciences, which enables investigators to describe the chemical composition of cells and tissues with high resolution. One of the major challenges of nanoSIMS is to identify specific molecules or organelles, as these are not immediately recognizable in nanoSIMS and need to be revealed by SIMS-compatible probes. Few laboratories have generated such probes, and none are commercially available. To address this, we performed a systematic study of probes initially developed for electron microscopy. Relying on nanoscale SIMS, we found that antibodies coupled to 6 nm gold particles are surprisingly efficient in terms of labeling specificity while offering a reliable detection threshold. These tools enabled accurate visualization and sample analysis and were easily employed in correlating SIMS with other imaging approaches, such as fluorescence microscopy. We conclude that antibodies conjugated to moderately sized gold particles are promising tools for SIMS imaging.

Keywords: nanoSIMS; mass spectrometry imaging; immunogold labeling; protein turnover

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MDPI and ACS Style

Li, F.; Fornasiero, E.F.; Dankovich, T.M.; Kluever, V.; Rizzoli, S.O. A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry. Int. J. Mol. Sci. 2022, 23, 4615. https://doi.org/10.3390/ijms23094615

AMA Style

Li F, Fornasiero EF, Dankovich TM, Kluever V, Rizzoli SO. A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry. International Journal of Molecular Sciences. 2022; 23(9):4615. https://doi.org/10.3390/ijms23094615

Chicago/Turabian Style

Li, Fengxia, Eugenio F. Fornasiero, Tal M. Dankovich, Verena Kluever, and Silvio O. Rizzoli. 2022. "A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry" International Journal of Molecular Sciences 23, no. 9: 4615. https://doi.org/10.3390/ijms23094615

APA Style

Li, F., Fornasiero, E. F., Dankovich, T. M., Kluever, V., & Rizzoli, S. O. (2022). A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry. International Journal of Molecular Sciences, 23(9), 4615. https://doi.org/10.3390/ijms23094615

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A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry

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