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Article

Efficient and Easy Conversion of Human iPSCs into Functional Induced Microglia-like Cells

1
Department of Stem Cell Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
2
Department of Medicine 1, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
3
Deutsches Zentrum Immuntherapie (DZI), 91054 Erlangen, Germany
4
Center of Rare Diseases Erlangen (ZSEER), Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
5
Department of Molecular Neurology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91054 Erlangen, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
These authors contributed equally to this work.
Academic Editors: Yohei Okada and Rivka Ofir
Int. J. Mol. Sci. 2022, 23(9), 4526; https://doi.org/10.3390/ijms23094526
Received: 10 February 2022 / Revised: 13 April 2022 / Accepted: 18 April 2022 / Published: 20 April 2022
(This article belongs to the Special Issue hiPSC-Derived Cells as Models for Drug Discovery 2.0)
Current protocols converting human induced pluripotent stem cells (iPSCs) into induced microglia-like cells (iMGL) are either dependent on overexpression of transcription factors or require substantial experience in stem-cell technologies. Here, we developed an easy-to-use two-step protocol to convert iPSCs into functional iMGL via: (1) highly efficient differentiation of hematopoietic progenitor cells (HPCs) from iPSCs, and (2) optimized maturation of HPCs to iMGL. A sequential harvesting approach led to an increased HPC yield. The protocol implemented a freezing step, thus allowing HPC biobanking and flexible timing of differentiation into iMGL. Our iMGL responded adequately to the inflammatory stimuli LPS, and iMGL RNAseq analysis matched those of other frequently used protocols. Comparing three different coating modalities, we increased the iMGL yield by culturing on uncoated glass surfaces, thereby retaining differentiation efficiency and functional hallmarks of iMGL. In summary, we provide a high-quality, easy-to-use protocol, rendering generation and functional studies on iMGL an accessible lab resource. View Full-Text
Keywords: microglia; pluripotent stem cells; cell differentiation; neurology; immunology microglia; pluripotent stem cells; cell differentiation; neurology; immunology
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MDPI and ACS Style

Lanfer, J.; Kaindl, J.; Krumm, L.; Gonzalez Acera, M.; Neurath, M.; Regensburger, M.; Krach, F.; Winner, B. Efficient and Easy Conversion of Human iPSCs into Functional Induced Microglia-like Cells. Int. J. Mol. Sci. 2022, 23, 4526. https://doi.org/10.3390/ijms23094526

AMA Style

Lanfer J, Kaindl J, Krumm L, Gonzalez Acera M, Neurath M, Regensburger M, Krach F, Winner B. Efficient and Easy Conversion of Human iPSCs into Functional Induced Microglia-like Cells. International Journal of Molecular Sciences. 2022; 23(9):4526. https://doi.org/10.3390/ijms23094526

Chicago/Turabian Style

Lanfer, Jonas, Johanna Kaindl, Laura Krumm, Miguel Gonzalez Acera, Markus Neurath, Martin Regensburger, Florian Krach, and Beate Winner. 2022. "Efficient and Easy Conversion of Human iPSCs into Functional Induced Microglia-like Cells" International Journal of Molecular Sciences 23, no. 9: 4526. https://doi.org/10.3390/ijms23094526

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