Melatonin Regulates Iron Homeostasis by Inducing Hepcidin Expression in Hepatocytes
Abstract
:1. Introduction
2. Results
2.1. Circadian Oscillation of Hepatic Hepcidin Gene Expression
2.2. Melatonin Induces Hepcidin Expression and Secretion
2.3. Melatonin Receptor Blockade Prevents Induction of Hepcidin by Melatonin
2.4. Melatonin Upregulates Hepcidin Expression by Activating the MT1-JNK-c-Jun Pathway
2.5. Melatonin Regulates Hepcidin Expression in Mice
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Plasmid DNAs
4.3. Animal Experiments
4.4. Cell Culture and Transient Transfection
4.5. RNA Interference
4.6. Q-PCR Analysis
4.7. Western Blot Analysis
4.8. Measurement of Hepcidin and Serum Iron Levels
4.9. ChIP Assay
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Park, W.-R.; Choi, B.; Kim, Y.-J.; Kim, Y.-H.; Park, M.-J.; Kim, D.-I.; Choi, H.-S.; Kim, D.-K. Melatonin Regulates Iron Homeostasis by Inducing Hepcidin Expression in Hepatocytes. Int. J. Mol. Sci. 2022, 23, 3593. https://doi.org/10.3390/ijms23073593
Park W-R, Choi B, Kim Y-J, Kim Y-H, Park M-J, Kim D-I, Choi H-S, Kim D-K. Melatonin Regulates Iron Homeostasis by Inducing Hepcidin Expression in Hepatocytes. International Journal of Molecular Sciences. 2022; 23(7):3593. https://doi.org/10.3390/ijms23073593
Chicago/Turabian StylePark, Woo-Ram, Byungyoon Choi, Yu-Ji Kim, Yong-Hoon Kim, Min-Jung Park, Dong-Il Kim, Hueng-Sik Choi, and Don-Kyu Kim. 2022. "Melatonin Regulates Iron Homeostasis by Inducing Hepcidin Expression in Hepatocytes" International Journal of Molecular Sciences 23, no. 7: 3593. https://doi.org/10.3390/ijms23073593