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Article

Self-Assembled Particles Combining SARS-CoV-2 RBD Protein and RBD DNA Vaccine Induce Synergistic Enhancement of the Humoral Response in Mice

1
State Research Center of Virology and Biotechnology “Vector”, 630559 Koltsovo, Novosibirsk Region, Russia
2
Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Science, 630090 Novosibirsk, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Evgenii Gusev
Int. J. Mol. Sci. 2022, 23(4), 2188; https://doi.org/10.3390/ijms23042188
Received: 28 January 2022 / Revised: 13 February 2022 / Accepted: 13 February 2022 / Published: 16 February 2022
Despite the fact that a range of vaccines against COVID-19 have already been created and are used for mass vaccination, the development of effective, safe, technological, and affordable vaccines continues. We have designed a vaccine that combines the recombinant protein and DNA vaccine approaches in a self-assembled particle. The receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 was conjugated to polyglucin:spermidine and mixed with DNA vaccine (pVAXrbd), which led to the formation of particles of combined coronavirus vaccine (CCV-RBD) that contain the DNA vaccine inside and RBD protein on the surface. CCV-RBD particles were characterized with gel filtration, electron microscopy, and biolayer interferometry. To investigate the immunogenicity of the combined vaccine and its components, mice were immunized with the DNA vaccine pVAXrbd or RBD protein as well as CCV-RBD particles. The highest antigen-specific IgG and neutralizing activity were induced by CCV-RBD, and the level of antibodies induced by DNA or RBD alone was significantly lower. The cellular immune response was detected only in the case of DNA or CCV-RBD vaccination. These results demonstrate that a combination of DNA vaccine and RBD protein in one construct synergistically increases the humoral response to RBD protein in mice. View Full-Text
Keywords: SARS-CoV-2; self-assembled particles; RBD protein; DNA vaccine; humoral response; virus-neutralizing activity; cellular response SARS-CoV-2; self-assembled particles; RBD protein; DNA vaccine; humoral response; virus-neutralizing activity; cellular response
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MDPI and ACS Style

Borgoyakova, M.B.; Karpenko, L.I.; Rudometov, A.P.; Volosnikova, E.A.; Merkuleva, I.A.; Starostina, E.V.; Zadorozhny, A.M.; Isaeva, A.A.; Nesmeyanova, V.S.; Shanshin, D.V.; Baranov, K.O.; Volkova, N.V.; Zaitsev, B.N.; Orlova, L.A.; Zaykovskaya, A.V.; Pyankov, O.V.; Danilenko, E.D.; Bazhan, S.I.; Shcherbakov, D.N.; Taranin, A.V.; Ilyichev, A.A. Self-Assembled Particles Combining SARS-CoV-2 RBD Protein and RBD DNA Vaccine Induce Synergistic Enhancement of the Humoral Response in Mice. Int. J. Mol. Sci. 2022, 23, 2188. https://doi.org/10.3390/ijms23042188

AMA Style

Borgoyakova MB, Karpenko LI, Rudometov AP, Volosnikova EA, Merkuleva IA, Starostina EV, Zadorozhny AM, Isaeva AA, Nesmeyanova VS, Shanshin DV, Baranov KO, Volkova NV, Zaitsev BN, Orlova LA, Zaykovskaya AV, Pyankov OV, Danilenko ED, Bazhan SI, Shcherbakov DN, Taranin AV, Ilyichev AA. Self-Assembled Particles Combining SARS-CoV-2 RBD Protein and RBD DNA Vaccine Induce Synergistic Enhancement of the Humoral Response in Mice. International Journal of Molecular Sciences. 2022; 23(4):2188. https://doi.org/10.3390/ijms23042188

Chicago/Turabian Style

Borgoyakova, Mariya B., Larisa I. Karpenko, Andrey P. Rudometov, Ekaterina A. Volosnikova, Iuliia A. Merkuleva, Ekaterina V. Starostina, Alexey M. Zadorozhny, Anastasiya A. Isaeva, Valentina S. Nesmeyanova, Daniil V. Shanshin, Konstantin O. Baranov, Natalya V. Volkova, Boris N. Zaitsev, Lyubov A. Orlova, Anna V. Zaykovskaya, Oleg V. Pyankov, Elena D. Danilenko, Sergei I. Bazhan, Dmitry N. Shcherbakov, Alexander V. Taranin, and Alexander A. Ilyichev. 2022. "Self-Assembled Particles Combining SARS-CoV-2 RBD Protein and RBD DNA Vaccine Induce Synergistic Enhancement of the Humoral Response in Mice" International Journal of Molecular Sciences 23, no. 4: 2188. https://doi.org/10.3390/ijms23042188

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