AKR1B10, One of the Triggers of Cytokine Storm in SARS-CoV2 Severe Acute Respiratory Syndrome
Abstract
:1. Introduction
2. Results
2.1. AKR1B10 Is Overexpressed in the Lung of Deceased COVID-19 Patients and Correlated with an Enrichment of Pro-Inflammatory and Cytokine Genes
2.2. AKR1B10 Levels Are Increased in the Blood of COVID-19 Patients with Severe or Critical Forms of the Disease Compared to Moderate Forms
2.3. AKR1B10 Drives Cytokine Production in Cellular Models
2.4. The AKR1B10 Protein Can Be Transferred between Cell Types via the Extracellular Vesicles
3. Discussion
Strength and Weakness
4. Materials and Methods
4.1. Sera
4.2. Cell Culture
4.3. RT-PCR
4.4. Western Blotting
4.5. Extracellular Vesicles (EVs) Extraction and Exposure
4.6. Transcriptomic Data Analysis
4.7. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Non-ICU | ICU | Adjusted p-Value | |||||
---|---|---|---|---|---|---|---|
Mean | ± | SEM | Mean | ± | SEM | ||
Sex (F/M) | 26/35 | 18/25 | |||||
Survival (% [Surv-deceased]) | 98.4% [60/1] | 37.2% [16/27] | <0.001 | ||||
Nb. of comorbidities: | |||||||
0 (n (%)) | 39 (63.9%) | 15 (34.9%) | <0.01 | ||||
1 (n (%)) | 12 (19.7%) | 16 (37.2%) | <0.05 | ||||
>=2 (n (%)) | 10 (16.4%) | 12 (27.9%) | 0.16 | ||||
Nature of comorbidities: | |||||||
Diabetes (n (%)) | 9 (15%) | 13 (30%) | 0.06 | ||||
HT (n (%)) | 16 (26%) | 15 (35%) | 0.35 | ||||
COPD (n (%)) | 2 (3%) | 4 (9%) | 0.20 | ||||
Renal disease (n (%)) | 1 (2%) | 2 (5%) | 0.37 | ||||
Cancer (n (%)) | 4 (7%) | 7 (16%) | 0.11 | ||||
Age (year) | 66.1 | ± | 8.51 | 71.6 | ± | 10.68 | 0.31 |
BMI (kg/m²) | 26.4 | ± | 3.66 | 26.8 | ± | 4.12 | 0.31 |
PaO2 (mmHg) | 59.9 | ± | 7.84 | 53.3 | ± | 8.21 | <0.01 |
CRP (mg/L) | 92.3 | ± | 10.58 | 138.1 | ± | 20.98 | 0.09 |
CT-Scan (%) | 33.3 | ± | 4.68 | 55.3 | ± | 8.22 | <0.01 |
Lymphocytes (abs. x10/µL) | 1.57 | ± | 0.17 | 0.86 | ± | 0.13 | <0.001 |
Fibrinogen (mg/dL) | 570.4 | ± | 73.54 | 588.5 | ± | 87.59 | 0.99 |
D.Dimer (ng/mL) | 2192 | ± | 286.02 | 3860 | ± | 560.52 | 0.31 |
Creatinine (mg/dL) | 1.41 | ± | 0.18 | 1.38 | ± | 0.21 | 0.64 |
Ferritin (ng/mL) | 546.0 | ± | 55.65 | 917.1 | ± | 152.55 | <0.05 |
LDH (UI/I) | 421.9 | ± | 54.75 | 528.6 | ± | 79.23 | 0.06 |
Procalcitonin (ng/mL) | 0.32 | ± | 0.04 | 1.01 | ± | 0.63 | 0.31 |
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Chabert, C.; Vitte, A.-L.; Iuso, D.; Chuffart, F.; Trocme, C.; Buisson, M.; Poignard, P.; Lardinois, B.; Debois, R.; Rousseaux, S.; et al. AKR1B10, One of the Triggers of Cytokine Storm in SARS-CoV2 Severe Acute Respiratory Syndrome. Int. J. Mol. Sci. 2022, 23, 1911. https://doi.org/10.3390/ijms23031911
Chabert C, Vitte A-L, Iuso D, Chuffart F, Trocme C, Buisson M, Poignard P, Lardinois B, Debois R, Rousseaux S, et al. AKR1B10, One of the Triggers of Cytokine Storm in SARS-CoV2 Severe Acute Respiratory Syndrome. International Journal of Molecular Sciences. 2022; 23(3):1911. https://doi.org/10.3390/ijms23031911
Chicago/Turabian StyleChabert, Clovis, Anne-Laure Vitte, Domenico Iuso, Florent Chuffart, Candice Trocme, Marlyse Buisson, Pascal Poignard, Benjamin Lardinois, Régis Debois, Sophie Rousseaux, and et al. 2022. "AKR1B10, One of the Triggers of Cytokine Storm in SARS-CoV2 Severe Acute Respiratory Syndrome" International Journal of Molecular Sciences 23, no. 3: 1911. https://doi.org/10.3390/ijms23031911