Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages
Abstract
:1. Introduction
2. Results
2.1. Effect of KW3110 on Mitochondrial Dysfunction in Inflammatory-Stressed J774A.1 Cells
2.2. KW3110 Reduces Mitochondrial Reactive Oxygen Species (ROS) Production in Inflammatory-Stressed J774A.1 Cells
2.3. Effect of KW3110 on Mitochondrial Respiratory Function in Inflammatory-Stressed J774A.1 Cells
2.4. IL-10 Suppresses Mitochondrial Dysfunction in Inflammatory-Stressed J774A.1 Cells
2.5. Effect of KW3110 and IL-10 on Disruption of Mitochondrial Morphology in Inflammatory-Stressed J774A.1 Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Mitochondrial Membrane Potential and ROS Analyses by Flow Cytometry
4.4. Mitochondrial Respiration Analysis
4.5. Determination of Cytokine Production
4.6. Transmission Electron Microscopy
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
LAB | lactic acid bacteria |
KW3110 | Lactobacillus paracasei KW3110 |
IL | interleukin |
LPS | lipopolysaccharide |
ROS | reactive oxygen species |
DMEM | Dulbecco’s modified Eagle medium |
PBS | phosphate-buffered saline |
OCR | oxygen consumption rate |
ELISA | enzyme-linked immunosorbent assay |
GA | glutaraldehyde |
PB | phosphate buffer |
RT | room temperature |
ANOVA | analysis of variance |
ATP | adenosine 5′-triphosphate |
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Yamazaki, T.; Yamada, S.; Ohshio, K.; Sugamata, M.; Morita, Y. Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages. Int. J. Mol. Sci. 2022, 23, 1443. https://doi.org/10.3390/ijms23031443
Yamazaki T, Yamada S, Ohshio K, Sugamata M, Morita Y. Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages. International Journal of Molecular Sciences. 2022; 23(3):1443. https://doi.org/10.3390/ijms23031443
Chicago/Turabian StyleYamazaki, Takahiro, Sayuri Yamada, Konomi Ohshio, Miho Sugamata, and Yuji Morita. 2022. "Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages" International Journal of Molecular Sciences 23, no. 3: 1443. https://doi.org/10.3390/ijms23031443