Easy and Effective Method for Extracting and Purifying Wolbachia Genomic DNA
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Drosophila Lines and Rearing
3.2. DNA Preparation and Sequencing
3.2.1. Wolbachia DNA Extraction from Drosophila Ovaries
3.2.2. Wolbachia DNA Extraction from a Whole Drosophila Imago
3.2.3. Genome Library Construction and Sequencing
3.3. Contamination, Quality Control and Trimming of the Sequenced Data
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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RefSeq Assembly Accession | Synonym | Species | Description |
---|---|---|---|
GCF_016584405.1 | wMelCS_b | Wolbachia endosymbiont of Drosophila melanogaster | Wolbachia reference genome |
GCF_000001215.4 | dm6 | Drosophila melanogaster | Release 6 plus ISO1 mitochondrial genome |
GCF_000001405.39 | hg38 | Homo sapiens | Genome Reference Consortium Human Build 38 patch release 13 (GRCh38.p13) |
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Andreenkova, O.V.; Shishkina, O.D.; Klimenko, A.I.; Korenskaia, A.E.; Bobrovskikh, M.A.; Shatskaya, N.V.; Vasiliev, G.V.; Gruntenko, N.E. Easy and Effective Method for Extracting and Purifying Wolbachia Genomic DNA. Int. J. Mol. Sci. 2022, 23, 15315. https://doi.org/10.3390/ijms232315315
Andreenkova OV, Shishkina OD, Klimenko AI, Korenskaia AE, Bobrovskikh MA, Shatskaya NV, Vasiliev GV, Gruntenko NE. Easy and Effective Method for Extracting and Purifying Wolbachia Genomic DNA. International Journal of Molecular Sciences. 2022; 23(23):15315. https://doi.org/10.3390/ijms232315315
Chicago/Turabian StyleAndreenkova, Olga V., Olga D. Shishkina, Alexandra I. Klimenko, Aleksandra E. Korenskaia, Margarita A. Bobrovskikh, Natalja V. Shatskaya, Gennady V. Vasiliev, and Nataly E. Gruntenko. 2022. "Easy and Effective Method for Extracting and Purifying Wolbachia Genomic DNA" International Journal of Molecular Sciences 23, no. 23: 15315. https://doi.org/10.3390/ijms232315315