Dihydrotanshinone I Inhibits the Lung Metastasis of Breast Cancer by Suppressing Neutrophil Extracellular Traps Formation
Abstract
:1. Introduction
2. Results
2.1. Tanshinones Inhibited Proliferation and Clonogenicity of BC Cells
2.2. DHT Inhibited the Migration and Invasion of 4T1 Cells
2.3. Induction of NETs and Its Correlation with BC
2.4. DHT Reversed NETs-Induced Proliferation, Migration, and ROS Production of 4T1 Cells
2.5. DHT Suppressed the NETs Formation by Restraining TIMP1 Expression
2.6. DHT Inhibited Tumor Growth and Lung Metastasis in 4T1 Tumor-Bearing Nude Mice
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture
4.3. MTT Assay
4.4. Colony Formation Assay
4.5. Wound Healing and Transwell Assays
4.6. Apoptosis Analysis
4.7. Extraction of PMNs and NETs
4.8. ROS Analysis
4.9. Hoechst 33342 Staining
4.10. dsDNA Test
4.11. Western Blotting
4.12. Animal Experiments
4.13. Hematoxylin and Eosin Staining
4.14. Immunofluorescence Analysis
4.15. Immunohistochemistry
4.16. Molecular Docking
4.17. TIMER Database Analysis
4.18. RNA Sequencing
4.19. Bioinformatics Analysis
4.20. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhao, H.; Liang, Y.; Sun, C.; Zhai, Y.; Li, X.; Jiang, M.; Yang, R.; Li, X.; Shu, Q.; Kai, G.; et al. Dihydrotanshinone I Inhibits the Lung Metastasis of Breast Cancer by Suppressing Neutrophil Extracellular Traps Formation. Int. J. Mol. Sci. 2022, 23, 15180. https://doi.org/10.3390/ijms232315180
Zhao H, Liang Y, Sun C, Zhai Y, Li X, Jiang M, Yang R, Li X, Shu Q, Kai G, et al. Dihydrotanshinone I Inhibits the Lung Metastasis of Breast Cancer by Suppressing Neutrophil Extracellular Traps Formation. International Journal of Molecular Sciences. 2022; 23(23):15180. https://doi.org/10.3390/ijms232315180
Chicago/Turabian StyleZhao, Huan, Yi Liang, Chengtao Sun, Yufei Zhai, Xuan Li, Mi Jiang, Ruiwen Yang, Xiaojuan Li, Qijin Shu, Guoyin Kai, and et al. 2022. "Dihydrotanshinone I Inhibits the Lung Metastasis of Breast Cancer by Suppressing Neutrophil Extracellular Traps Formation" International Journal of Molecular Sciences 23, no. 23: 15180. https://doi.org/10.3390/ijms232315180