Mlig-SKP1 Gene Is Required for Spermatogenesis in the Flatworm Macrostomum lignano
Abstract
:1. Introduction
2. Results and Discussion
2.1. Knockdown of Mlig-SKP1 Gene Does Not Affect Regeneration but Causes Abnormal Testes Morphology
2.2. Mlig-SKP1 Is Expressed in Gonads
2.3. Mlig-SKP1 Knockdown Leads to Decreased Fertility
2.4. Mlig-SKP1 Knockdown Results in Aberrant Morphology of Sperm Cells
2.5. Conserved Role of SKP1 in Regulation of Spermatogenesis
3. Materials and Methods
3.1. Organism and Its Culture Conditions
3.2. RNA Interference
3.3. Whole-Mount In Situ Hybridization
3.4. RNAi Fertility Experiments
3.5. qRT-PCR for Knock Down Verification
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Biryukov, M.; Dmitrieva, A.; Vavilova, V.; Ustyantsev, K.; Bazarova, E.; Sukhikh, I.; Berezikov, E.; Blinov, A. Mlig-SKP1 Gene Is Required for Spermatogenesis in the Flatworm Macrostomum lignano. Int. J. Mol. Sci. 2022, 23, 15110. https://doi.org/10.3390/ijms232315110
Biryukov M, Dmitrieva A, Vavilova V, Ustyantsev K, Bazarova E, Sukhikh I, Berezikov E, Blinov A. Mlig-SKP1 Gene Is Required for Spermatogenesis in the Flatworm Macrostomum lignano. International Journal of Molecular Sciences. 2022; 23(23):15110. https://doi.org/10.3390/ijms232315110
Chicago/Turabian StyleBiryukov, Mikhail, Anastasia Dmitrieva, Valeriya Vavilova, Kirill Ustyantsev, Erzhena Bazarova, Igor Sukhikh, Eugene Berezikov, and Alexandr Blinov. 2022. "Mlig-SKP1 Gene Is Required for Spermatogenesis in the Flatworm Macrostomum lignano" International Journal of Molecular Sciences 23, no. 23: 15110. https://doi.org/10.3390/ijms232315110