Dihydromyricetin Inhibited Migration and Invasion by Reducing S100A4 Expression through ERK1/2/β-Catenin Pathway in Human Cervical Cancer Cell Lines
Abstract
:1. Introduction
2. Results
2.1. Cell Viability and Cell Migration and Invasion of DHM on Cervical Cancer Cell Lines
2.2. DHM Reduced S100A4 Gene Expression in Cervical Cancer Cells
2.3. DHM Inhibited Cell Migration by Reducing S100A4 Expression through ERK1/2/β-Catenin Pathway
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Culture
4.2. Cell Viability Assay
4.3. Wound Healing Assay
4.4. Quantitative Real-Time PCR
4.5. Cell Migration and Invasion Assay
4.6. Western Blot Assay
4.7. CS2-S100A4 Transfection
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hsin, M.-C.; Hsiao, Y.-H.; Chen, P.-N.; Lin, C.-W.; Wang, P.-H.; Yang, S.-F.; Lee, C.-Y. Dihydromyricetin Inhibited Migration and Invasion by Reducing S100A4 Expression through ERK1/2/β-Catenin Pathway in Human Cervical Cancer Cell Lines. Int. J. Mol. Sci. 2022, 23, 15106. https://doi.org/10.3390/ijms232315106
Hsin M-C, Hsiao Y-H, Chen P-N, Lin C-W, Wang P-H, Yang S-F, Lee C-Y. Dihydromyricetin Inhibited Migration and Invasion by Reducing S100A4 Expression through ERK1/2/β-Catenin Pathway in Human Cervical Cancer Cell Lines. International Journal of Molecular Sciences. 2022; 23(23):15106. https://doi.org/10.3390/ijms232315106
Chicago/Turabian StyleHsin, Min-Chieh, Yi-Hsuan Hsiao, Pei-Ni Chen, Chiao-Wen Lin, Po-Hui Wang, Shun-Fa Yang, and Chung-Yuan Lee. 2022. "Dihydromyricetin Inhibited Migration and Invasion by Reducing S100A4 Expression through ERK1/2/β-Catenin Pathway in Human Cervical Cancer Cell Lines" International Journal of Molecular Sciences 23, no. 23: 15106. https://doi.org/10.3390/ijms232315106