Different Effects of Different Lactobacillus acidophilus Strains on DSS-Induced Colitis
Abstract
:1. Introduction
2. Results
2.1. L. acidophilus Improved the Symptoms of Mice with Colitis
2.2. L. acidophilus Affected the Cytokines in the Colon of Mice
2.3. L. acidophilus Affected the CCL2/CCR2 Axis and CCL3/CCR1 Axis Downstream IL-17 Signal Pathway in the Colon of Mice
2.4. L. acidophilus Modified the Diversity of Gut Microbiota in DSS-Induced Colitis Mice
2.5. L. acidophilus Modified the Composition of Gut Microbiota in DSS-Induced Colitis Mice
2.6. PICRUSt Analysis of Gut Microbiota in DSS-Induced Colitis Mice Modified by L. acidophilus
2.7. L. acidophilus Modified the Fecal SCFA in DSS-Induced Colitis Mice
2.8. Genome Analysis of L. acidophilus
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains and Preparation
4.2. Animal Trial Design
4.3. Assessment of Colitis Symptoms
4.4. Biochemical Assays
4.5. RNA Isolation and Real-Time Quantitative-PCR
4.6. DNA Extraction and Pyrosequencing
4.7. Short Chain Fatty Acid Determination
4.8. Genome Analysis
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer | Sequence |
---|---|
CCL2-F | 5′-TTAAAAACCTGGATCGGAACCAA-3′ |
CCL2-R | 5′-GCATTAGCTTCAGATTTACGGGT-3′ |
CCL3-F | 5′-TTCTCTGTACCATGACACTCTGC-3′ |
CCL3-R | 5′-CGTGGAATCTTCCGGCTGTAG-3′ |
CCR2-F | 5′-ATCCACGGCATACTATCAACATC-3′ |
CCR2-R | 5′-CAAGGCTCACCATCATCGTAG-3′ |
CCR1-F | 5′-CTCATGCAGCATAGGAGGCTT-3′ |
CCR1-R | 5′-ACATGGCATCACCAAAAATCCA-3′ |
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Huang, Z.; Gong, L.; Jin, Y.; Stanton, C.; Ross, R.P.; Zhao, J.; Yang, B.; Chen, W. Different Effects of Different Lactobacillus acidophilus Strains on DSS-Induced Colitis. Int. J. Mol. Sci. 2022, 23, 14841. https://doi.org/10.3390/ijms232314841
Huang Z, Gong L, Jin Y, Stanton C, Ross RP, Zhao J, Yang B, Chen W. Different Effects of Different Lactobacillus acidophilus Strains on DSS-Induced Colitis. International Journal of Molecular Sciences. 2022; 23(23):14841. https://doi.org/10.3390/ijms232314841
Chicago/Turabian StyleHuang, Zheng, Lei Gong, Yan Jin, Catherine Stanton, Reynolds Paul Ross, Jianxin Zhao, Bo Yang, and Wei Chen. 2022. "Different Effects of Different Lactobacillus acidophilus Strains on DSS-Induced Colitis" International Journal of Molecular Sciences 23, no. 23: 14841. https://doi.org/10.3390/ijms232314841