Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary
Abstract
:1. Introduction
2. Results
2.1. MAO Shows Its Activity in Granulosa Cells of Growing Follicles
2.2. Granulosa Cells Uptake 5-HT via SERT Activity
2.3. Granulosa Cells of Growing Follicles Act as a Functional Barrier for 5-HT
3. Discussion
4. Materials and Methods
4.1. Experimental Animals and Chemicals
4.2. Ovary Fragments and Denuded Oocytes Incubation Experiments
4.3. Granulosa Cells Incubation Experiments
4.4. Immunohistochemistry
4.5. Image Analysis and Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Alyoshina, N.M.; Tkachenko, M.D.; Malchenko, L.A.; Shmukler, Y.B.; Nikishin, D.A. Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary. Int. J. Mol. Sci. 2022, 23, 14828. https://doi.org/10.3390/ijms232314828
Alyoshina NM, Tkachenko MD, Malchenko LA, Shmukler YB, Nikishin DA. Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary. International Journal of Molecular Sciences. 2022; 23(23):14828. https://doi.org/10.3390/ijms232314828
Chicago/Turabian StyleAlyoshina, Nina M., Maria D. Tkachenko, Lyudmila A. Malchenko, Yuri B. Shmukler, and Denis A. Nikishin. 2022. "Uptake and Metabolization of Serotonin by Granulosa Cells Form a Functional Barrier in the Mouse Ovary" International Journal of Molecular Sciences 23, no. 23: 14828. https://doi.org/10.3390/ijms232314828