The Genome of the Mitochondrion-Related Organelle in Cepedea longa, a Large Endosymbiotic Opalinid Inhabiting the Recta of Frogs
Abstract
:1. Introduction
2. Results
2.1. Cepedea longa and Its MRO Architecture
2.2. Comparison of MRO Genome Features in Opalinata Species
2.3. Phylogenetic Analyses
3. Discussion
4. Materials and Methods
4.1. Specimen Collection, Identification and Observation
4.2. DNA Extraction, MRO Genome Sequencing and Assembly
4.3. MRO Genome Annotation and Analysis
4.4. Phylogenetic Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cepedea longa | Proteromonas lacertae | Blastocystis sp. NandII | Blastocystis sp. Strain Flemming | Blastocystis sp. Strain F5323 | |
---|---|---|---|---|---|
Total length (bp) | 62,395 | 48,663 | 28,385 | 28,305 | 28,788 |
Total ORFs length (bp) | 38,313 | 34,569 | 21,966 | 21,594 | 21,909 |
Total intergenic regions length (bp) | 11,510 | 2340 | 1162 | 1046 | 1225 |
G + C content (%) | |||||
Total | 25.7 | 22.7 | 19.9 | 19.7 | 18.9 |
Intergenic regions (IGRs) | 25.4 | 12.0 | 10.8 | 11.9 | 8.4 |
Protein-coding regions | 23.3 | 19.0 | 17.9 | 17.8 | 17.1 |
Structural RNA genes | 34.6 | 35.4 | 30.0 | 29.4 | 29.0 |
Gene number | |||||
Protein coding | 40 | 39 | 27 | 26 | 26 |
Structural RNAs | 45 | 49 | 18 | 18 | 17 |
Average ORFs length (bp) | 958 | 886 | 813 | 830 | 843 |
Average IGRs length (bp) | 151 | 31 | 33 | 29 | 40 |
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Zhao, W.; Bu, X.; Zou, H.; Li, W.; Wu, S.; Li, M.; Wang, G. The Genome of the Mitochondrion-Related Organelle in Cepedea longa, a Large Endosymbiotic Opalinid Inhabiting the Recta of Frogs. Int. J. Mol. Sci. 2022, 23, 13472. https://doi.org/10.3390/ijms232113472
Zhao W, Bu X, Zou H, Li W, Wu S, Li M, Wang G. The Genome of the Mitochondrion-Related Organelle in Cepedea longa, a Large Endosymbiotic Opalinid Inhabiting the Recta of Frogs. International Journal of Molecular Sciences. 2022; 23(21):13472. https://doi.org/10.3390/ijms232113472
Chicago/Turabian StyleZhao, Weishan, Xialian Bu, Hong Zou, Wenxiang Li, Shangong Wu, Ming Li, and Guitang Wang. 2022. "The Genome of the Mitochondrion-Related Organelle in Cepedea longa, a Large Endosymbiotic Opalinid Inhabiting the Recta of Frogs" International Journal of Molecular Sciences 23, no. 21: 13472. https://doi.org/10.3390/ijms232113472