Preventing Antibiotic-Resistant Infections: Additively Manufactured Porous Ti6Al4V Biofunctionalized with Ag and Fe Nanoparticles
Abstract
:1. Introduction
2. Results
2.1. Surface Biofunctionalization of Ti6Al4V Scaffolds
2.2. Ag, Fe, and Ca Ion Release Kinetics
2.3. Antibacterial Properties against P. aeruginosa and MRSA
2.3.1. Zone of Inhibition
2.3.2. Bactericidal Activity
2.4. Cytocompatibility
2.4.1. Cell Proliferation and Morphology
2.4.2. Immunostaining of Phospho-Calcium Sensing Receptors
3. Discussion
4. Materials and Methods
4.1. Scaffold Design and Selective Laser Melting
4.2. Plasma Electrolytic Oxidation (PEO)
4.3. Characterization of Surface Morphology, Porosity, and Phase Composition
4.4. Release of Ag, Fe, and Ca Ions
4.5. Antibacterial Assays
4.5.1. Agar Diffusion
4.5.2. Quantitative Bactericidal Activity
4.6. Cytocompatibility
4.6.1. Preculture of Cells and Cell Seeding
4.6.2. Live/Dead Staining and SEM Imaging
4.6.3. Immunostaining
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Specimen Group | Calcium Acetate (M) | Calcium Glycerophosphate (M) | Ag NPs (g/L) | Fe NPs (g/L) |
---|---|---|---|---|
PEO | 0.15 | 0.2 | - | - |
PEO Ag3 | 0.15 | 0.2 | 3 | - |
PEO Ag2Fe | 0.15 | 0.2 | 1.5 | 0.5 |
PEO Ag1Fe | 0.15 | 0.2 | 1 | 0.5 |
PEO Fe | 0.15 | 0.2 | - | 0.5 |
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Putra, N.E.; Leeflang, M.A.; Ducret, V.; Patrulea, V.; Fratila-Apachitei, L.E.; Perron, K.; Ye, H.; Zhou, J.; Apachitei, I.; Zadpoor, A.A. Preventing Antibiotic-Resistant Infections: Additively Manufactured Porous Ti6Al4V Biofunctionalized with Ag and Fe Nanoparticles. Int. J. Mol. Sci. 2022, 23, 13239. https://doi.org/10.3390/ijms232113239
Putra NE, Leeflang MA, Ducret V, Patrulea V, Fratila-Apachitei LE, Perron K, Ye H, Zhou J, Apachitei I, Zadpoor AA. Preventing Antibiotic-Resistant Infections: Additively Manufactured Porous Ti6Al4V Biofunctionalized with Ag and Fe Nanoparticles. International Journal of Molecular Sciences. 2022; 23(21):13239. https://doi.org/10.3390/ijms232113239
Chicago/Turabian StylePutra, Niko E., Marius A. Leeflang, Verena Ducret, Viorica Patrulea, Lidy E. Fratila-Apachitei, Karl Perron, Hua Ye, Jie Zhou, Iulian Apachitei, and Amir A. Zadpoor. 2022. "Preventing Antibiotic-Resistant Infections: Additively Manufactured Porous Ti6Al4V Biofunctionalized with Ag and Fe Nanoparticles" International Journal of Molecular Sciences 23, no. 21: 13239. https://doi.org/10.3390/ijms232113239