Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Phylogenetic Analysis of Quinoa CqKCS
2.2. Expression Pattern of CqKCS2 in C. Quinoa
2.3. Localization of CqKCS2B.1 in the Endoplasmic Reticulum
2.4. Ectopic Expression of CqKCS2B.1 Promotes Lateral Root Development in Arabidopsis
2.5. CqKCSB.1 Overexpression Alters Composition of Suberin Monomers in Arabidopsis Roots
2.6. Ectopic Expression of CqKCS2B.1 Increases the Salt Stress Tolerance of Arabidopsis
3. Discussion
4. Materials and Methods
4.1. Identification of CqKCS Genes
4.2. Phylogenetic Analysis
4.3. Gene Duplication Analysis
4.4. Plant Materials and Growth Conditions
4.5. RNA Extraction, RT-PCR and RT-qPCR Analysis
4.6. Subcellular Localization of CqKCS2B.1
4.7. Generation of CqKCS2B.1 Overexpression Lines in Arabidopsis
4.8. Determination of Salt Tolerance of WT and CqKCS2B.1 OE Lines
4.9. Suberin Content Analysis in Plant Root
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tariq, F.; Zhao, S.; Ahmad, N.; Wang, P.; Shao, Q.; Ma, C.; Yang, X. Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana. Int. J. Mol. Sci. 2022, 23, 13204. https://doi.org/10.3390/ijms232113204
Tariq F, Zhao S, Ahmad N, Wang P, Shao Q, Ma C, Yang X. Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana. International Journal of Molecular Sciences. 2022; 23(21):13204. https://doi.org/10.3390/ijms232113204
Chicago/Turabian StyleTariq, Faheem, Shuangshuang Zhao, Naveed Ahmad, Pingping Wang, Qun Shao, Changle Ma, and Xianpeng Yang. 2022. "Overexpression of β-Ketoacyl CoA Synthase 2B.1 from Chenopodium quinoa Promotes Suberin Monomers’ Production and Salt Tolerance in Arabidopsis thaliana" International Journal of Molecular Sciences 23, no. 21: 13204. https://doi.org/10.3390/ijms232113204