ENO1 Binds to ApoC3 and Impairs the Proliferation of T Cells via IL-8/STAT3 Pathway in OSCC
Abstract
:1. Introduction
2. Results
2.1. ENO1 Was Overexpressed in Human OSCC Tissues
2.2. Clinicopathological Correlation of ENO1 Expression Level in OSCC
2.3. ENO1 Directly Interacted with ApoC3 in OSCC
2.4. ApoC3 was Overexpressed in Human OSCC
2.5. ENO1 Binding to ApoC3 Mediated the Production of Proinflammatory Cytokines
2.6. IL-8 Impaired the Proliferation of T Cells through the STAT3 Signaling Pathway
3. Discussion
4. Materials and Methods
4.1. Patient and Sample Collection
4.2. Human OSCC Tissue Microarrays
4.3. Immunohistochemical (IHC) Staining
4.4. Human Lymphatic Drainage
4.5. GST Pulldown Assay
4.6. Mass Spectrometry
4.7. KEGG Pathway Enrichment and PPI Network Analysis
4.8. Cell Culture
4.9. Immunofluorescence
4.10. Confocal Microscopy
4.11. Flow Cytometry
4.12. Co-Culture of Jurkat T and CAL27 Cells
4.13. SiRNA Knockdown of ENO1
4.14. Western Blot Analysis
4.15. Inflammatory Cytokine Assay
4.16. Enzyme-Linked Immunosorbent Assay (ELISA)
4.17. Cell Cytotoxicity Assay
4.18. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Accession | Gene | Identified Proteins | Coverage | Peptides |
---|---|---|---|---|
P02042 | HBD | Hemoglobin subunit delta | 70.07 | 37 |
A0A0C4DH69 | IGKV1-9 | Immunoglobulin kappa variable 1-9 | 48.72 | 5 |
A0A0C4DH38 | IGHV5-51 | Immunoglobulin heavy variable 5-51 | 45.30 | 6 |
P02656 | APOC3 | Apolipoprotein C-III | 44.44 | 3 |
P01611 | IGKV1D-12 | Immunoglobulin kappa variable 1D-12 | 43.59 | 7 |
P09382 | LGALS1 | Galectin-1 | 42.21 | 4 |
A0A087WSY6 | IGKV3D-15 | Immunoglobulin kappa variable 3D-15 | 40.00 | 8 |
P00915 | CA1 | Carbonic anhydrase 1 | 35.62 | 7 |
P61626 | LYZ | Lysozyme C | 35.13 | 3 |
P06312 | IGKV4-1 | Immunoglobulin kappa variable 4-1 | 34.70 | 7 |
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Wang, J.; Man, Q.; Zhong, N.; Wang, H.; Zhang, C.; Li, S.; Bu, L.; Liu, B. ENO1 Binds to ApoC3 and Impairs the Proliferation of T Cells via IL-8/STAT3 Pathway in OSCC. Int. J. Mol. Sci. 2022, 23, 12777. https://doi.org/10.3390/ijms232112777
Wang J, Man Q, Zhong N, Wang H, Zhang C, Li S, Bu L, Liu B. ENO1 Binds to ApoC3 and Impairs the Proliferation of T Cells via IL-8/STAT3 Pathway in OSCC. International Journal of Molecular Sciences. 2022; 23(21):12777. https://doi.org/10.3390/ijms232112777
Chicago/Turabian StyleWang, Jing, Qiwen Man, Niannian Zhong, Hanqi Wang, Chenxi Zhang, Suran Li, Linlin Bu, and Bing Liu. 2022. "ENO1 Binds to ApoC3 and Impairs the Proliferation of T Cells via IL-8/STAT3 Pathway in OSCC" International Journal of Molecular Sciences 23, no. 21: 12777. https://doi.org/10.3390/ijms232112777