Recombinant Human Proteoglycan 4 (rhPRG4) Downregulates TNFα-Stimulated NFκB Activity and FAT10 Expression in Human Corneal Epithelial Cells
Abstract
:1. Introduction
2. Results
2.1. rhPRG4 Reduces TNFα-Mediated Inflammation in hTCEpi Cells
2.2. rhPRG4 Reduces TNFα-Stimulated p65 Phosphorylation in hTCEpi Cells
2.3. Identifying TNFα-Activated Genes Specifically Reversed in Expression by rhPRG4
2.4. rhPRG4 Reduces TNFα-Stimulated FAT10 RNA and Protein Expression
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.1.1. Cell Treatment and Sample Collection—Time Course for NFκB Activity and Translocation
4.1.2. Cell Treatment and Sample Collection—Single Timepoint for RNA and FAT10 Analysis
4.2. Western Blotting
4.3. RNA Sequencing & Bioinformatics
4.4. Immunofluorescence
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Menon, N.G.; Suhail, Y.; Goyal, R.; Du, W.; Tanguay, A.P.; Jay, G.D.; Ghosh, M.; Kshitiz; Schmidt, T.A. Recombinant Human Proteoglycan 4 (rhPRG4) Downregulates TNFα-Stimulated NFκB Activity and FAT10 Expression in Human Corneal Epithelial Cells. Int. J. Mol. Sci. 2022, 23, 12711. https://doi.org/10.3390/ijms232112711
Menon NG, Suhail Y, Goyal R, Du W, Tanguay AP, Jay GD, Ghosh M, Kshitiz, Schmidt TA. Recombinant Human Proteoglycan 4 (rhPRG4) Downregulates TNFα-Stimulated NFκB Activity and FAT10 Expression in Human Corneal Epithelial Cells. International Journal of Molecular Sciences. 2022; 23(21):12711. https://doi.org/10.3390/ijms232112711
Chicago/Turabian StyleMenon, Nikhil G., Yasir Suhail, Ruchi Goyal, Wenqiang Du, Adam P. Tanguay, Gregory D. Jay, Mallika Ghosh, Kshitiz, and Tannin A. Schmidt. 2022. "Recombinant Human Proteoglycan 4 (rhPRG4) Downregulates TNFα-Stimulated NFκB Activity and FAT10 Expression in Human Corneal Epithelial Cells" International Journal of Molecular Sciences 23, no. 21: 12711. https://doi.org/10.3390/ijms232112711