Nutritional Approaches as a Treatment for Impaired Bone Growth and Quality Following the Consumption of Ultra-Processed Food
Abstract
:1. Introduction
2. Results
2.1. Analysis of the Macro and Micro-Nutrient Content of the Control and UPF
2.2. The Effect of Micro-Nutrient Composition on Growth and Physiological Parameters
2.2.1. The Effect of UPF and Supplementations on the Metabolic Profile of the Rats
2.2.2. The Effect of UPF and Supplementations on the Rats’ Bone Properties
2.2.3. The Effect of Supplementations with Calcium or Copper on Growth and Metabolic Status
2.2.4. The Effect of Ca or Cu Supplementations on Rat Bone Properties
2.2.5. The Effect of the Different Diets and Supplementations on the Kidneys State
2.2.6. Rescue Experiment: Short or Prolonged Transition to Balanced Nutrition
3. Discussion
4. Materials and Methods
4.1. Experimental Design
- Control diet: 20% protein, 16% fat and 64% carbohydrate.
- Ultra-processed diet: 20% protein, 40% fat and 40% carbohydrate.
- Ultra-processed diet with multivitamin and mineral supplement: 20% protein, 40% fat and 40% carbohydrate; supplemented with multi vitamins and minerals (AIN-93-VX Vitamin Mix and AIN Mineral Mixture 76), while the Ca content was the guideline for supplementation and equal to the Control.
- Ultra-processed diet with Ca supplement: 20% protein, 40% fat and 40% carbohydrate; supplemented with Ca phosphate according to the calcium content of the Control chew diet.
- Ultra-processed diet with copper supplement: 20% protein, 40% fat and 40% carbohydrate; supplemented with copper carbonate basic according to Harlan’s copper content of the Control chew diet.
4.2. Diet Analysis
4.3. Blood Analysis
4.4. Histological Analyses of the Growth Plate and Kidneys
4.5. Skeleton Analyses
4.5.1. Micro Computed Tomography (µCT) Analysis
4.5.2. Light Microscopy Images of Cross-Sections of Rat Cortical Bone
4.5.3. Mechanical Testing
4.6. RNA Isolation, Reverse Transcription, and Real-Time PCR
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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The Tested Parameter | Calcium Supplementation Experiment | Copper Supplementation Experiment | ||||
---|---|---|---|---|---|---|
Trabeculacr analysis of the femur | Control | UPF + CSD | UPF + CSD + Ca | Control | UPF | UPF + Cu |
BV/TV (%) | 27.37 ± 4.61 a | 16.61 ± 3.85 b | 24.86 ± 2.73 ab | 31.26 ± 3.33 a | 18.47 ± 3.82 b | 18.07 ± 2.54 b |
Tb.N (1/mm) | 2.41 ± 0.25 a | 1.76 ± 0.40 b | 2.07 ± 0.19 ab | 2.65 ± 0.24 a | 2.27 ± 0.41 b | 2.23 ± 0.39 b |
Tb.Th (mm) | 0.11 ± 0.008 ab | 0.09 ± 0.003 b | 0.12 ± 0.006 a | 0.12 ± 0.004 a | 0.08 ± 0.003 b | 0.08 ± 0.01 b |
Tb.Sp (mm) | 0.31 ± 0.038 b | 0.53 ± 0.18 a | 0.42 ± 0.09 b | 0.28 ± 0.03 b | 0.56 ± 0.17 a | 0.43 ± 0.02 a |
Trabeculacr analysis of the vertebra | Control | UPF + CSD | UPF + CSD + Ca | Control | UPF | UPF + Cu |
BV/TV (%) | 40.37 ± 3.78 a | 26.50 ± 3.55 c | 34.05 ± 2.48 b | 42.9 ± 2.82 a | 21.06 ± 1.63 b | 22.62 ± 2.96 b |
Tb.N (1/mm) | 4.73 ± 0.58 a | 3.53 ± 0.42 b | 4.03 ± 0.28 b | 2.87 ± 0.14 a | 1.97 ± 0.11 b | 2.00 ± 0.16 b |
Tb.Th (mm) | 0.086 ± 0.003 a | 0.075 ± 0.004 b | 0.085 ± 0.006 a | 0.15 ± 0.01 a | 0.11 ± 0.003 b | 0.11 ± 0.01 b |
Tb.Sp (mm) | 0.23 ± 0.03 b | 0.30 ± 0.03 a | 0.27 ± 0.01 a | 0.26 ± 0.02 b | 0.35 ± 0.02 a | 0.35 ± 0.02 a |
Cortical analysis of the femur | Control | UPF + CSD | UPF + CSD + Ca | Control | UPF | UPF + Cu |
Ct.Ar/Tt.Ar (%) | 46.62 ± 3.93 b | 31.34 ± 3.87 c | 51.29 ± 2.12 a | 48.61 ± 2.23 a | 33.59 ± 3.72 b | 36.23 ± 3.17 b |
Ct.Th (mm) | 0.47 ± 0.03 a | 0.24 ± 0.02 b | 0.50 ± 0.02 a | 0.44 ± 0.02 a | 0.23 ± 0.02 c | 0.26 ± 0.02 b |
Ma.Ar (mm2) | 6.07 ± 0.7 a | 6.67 ± 0.95 a | 4.91 ± 0.37 b | 4.63 ± 0.39 b | 5.34 ± 0.71 a | 5.12 ± 0.29 ab |
BMD (g/cm3) | 1.10 ± 0.02 b | 0.81 ± 0.05 c | 1.17 ± 0.01 a | 1.54 ± 0.03 a | 1.08 ± 0.04 b | 1.03 ± 0.04 c |
Ct.Po (%) | 0.06 ± 0.05 b | 2.3 ± 0.83 a | 0.02 ± 0.02 b | 0.13 ± 0.10 c | 4.61 ± 1.29 a | 3.30 ± 1.15 b |
Po.N (n) | 3.83 ± 2.14 b | 21 ± 7.42 a | 1.83 ± 0.98 b | 1.6 ± 1.07 b | 34.45 ± 17.10 a | 28.00 ± 14.35 a |
Po.V (mm3) | 0.01 ± 0.01 b | 0.2 ± 0.1 a | 0.003 ± 0.003 b | 0.02 ± 0.01 b | 0.33 ± 0.10 a | 0.26 ± 0.09 a |
Three point bending test | Control | UPF + CSD | UPF + CSD + Ca | Control | UPF | UPF + Cu |
Stiffness (N/mm) | 239.56 ± 45.30 a | 36.18 ± 14.38 b | 282.44 ± 39.52 a | 273.77 ± 23.53 a | 51.59 ± 13.70 b | 40.64 ± 7.88 b |
yield (N) | 49.49 ± 3.07 a | 17.47 ± 2.81 b | 50.94 ± 6.88 a | 42.02 ± 4.38 a | 14.02 ± 2.50 b | 14.77 ± 1.78 b |
fracture load (N) | 72.00 ± 10.14 a | 12.86 ± 7.28 b | 71.56 ± 12.52 a | 74.95 ± 10.08 a | 18.11 ± 6.65 b | 16.78 ± 6.66 b |
Max load (N) | 79.31 ± 8.40 a | 22.03 ± 3.45 b | 82.18 ± 8.04 a | 87.04 ± 8.16 a | 25.28 ± 3.76 b | 23.35 ± 3.33 b |
Energy to fracture (Nxmm) | 54.29 ± 7.39 a | 36.14 ± 11.58 b | 50.23 ± 7.92 a | 66.57 ± 14.35 a | 40.86 ± 7.82 b | 41.35 ± 6.50 b |
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Griess-Fishheimer, S.; Zaretsky, J.; Travinsky-Shmul, T.; Zaretsky, I.; Penn, S.; Shahar, R.; Monsonego-Ornan, E. Nutritional Approaches as a Treatment for Impaired Bone Growth and Quality Following the Consumption of Ultra-Processed Food. Int. J. Mol. Sci. 2022, 23, 841. https://doi.org/10.3390/ijms23020841
Griess-Fishheimer S, Zaretsky J, Travinsky-Shmul T, Zaretsky I, Penn S, Shahar R, Monsonego-Ornan E. Nutritional Approaches as a Treatment for Impaired Bone Growth and Quality Following the Consumption of Ultra-Processed Food. International Journal of Molecular Sciences. 2022; 23(2):841. https://doi.org/10.3390/ijms23020841
Chicago/Turabian StyleGriess-Fishheimer, Shelley, Janna Zaretsky, Tamara Travinsky-Shmul, Irina Zaretsky, Svetlana Penn, Ron Shahar, and Efrat Monsonego-Ornan. 2022. "Nutritional Approaches as a Treatment for Impaired Bone Growth and Quality Following the Consumption of Ultra-Processed Food" International Journal of Molecular Sciences 23, no. 2: 841. https://doi.org/10.3390/ijms23020841