Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 CYP4BQ1 Responsible for (+)-α-Pinene Tolerance in Dendroctonus armandi
Abstract
:1. Introduction
2. Results
2.1. (+)-α-Pinene Exposure Induces DaCYP4BQ1 Expression
2.2. Spatiotemporal Expression Pattern of CYP4BQ1
2.3. CYP4BQ1 Participates in Adults’ Tolerance to (+)-α-Pinene
2.4. (+)-α-Pinene Exposure Induces Expression of CncC and Maf
2.5. Silencing CncC Suppresses CYP4BQ1 Expression and Reduces Adults’ Tolerance to (+)-α-Pinene
2.6. (+)-α-Pinene Exposure Enhances H2O2 Generation and the Activities of Antioxidant Enzymes
2.7. Scavenging of ROS Downregulates CncC and CYP4BQ1 Expressions and Reduces Adults’ Tolerance to (+)-α-Pinene
2.8. The CncC Agonist Curcumin Induces the Expression of CYP4BQ1 and Enhances Adults’ Tolerance to (+)-α-Pinene
3. Discussion
4. Materials and Methods
4.1. Insects and Reagents Preparation
4.2. Tolerance to (+)-α-Pinene
4.3. RNA Extraction, cDNA Synthesis, and Reverse Transcription Quantitative PCR (RT-qPCR)
4.4. Developmental and Tissue-Dependent Expression Profiles of CYP4BQ1, CncC, Maf, and Keap1
4.5. Double-Strand RNA (dsRNA) Synthesis and Injection
4.6. H2O2 Content and CAT, SOD and POD Activity Determination
4.7. Scavenging of ROS by N-Acetylcysteine (NAC) Treatment
4.8. Dietary Curcumin and (+)-α-Pinene Tolerance Analyses
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, B.; Tang, M.; Chen, H. Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 CYP4BQ1 Responsible for (+)-α-Pinene Tolerance in Dendroctonus armandi. Int. J. Mol. Sci. 2022, 23, 11578. https://doi.org/10.3390/ijms231911578
Liu B, Tang M, Chen H. Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 CYP4BQ1 Responsible for (+)-α-Pinene Tolerance in Dendroctonus armandi. International Journal of Molecular Sciences. 2022; 23(19):11578. https://doi.org/10.3390/ijms231911578
Chicago/Turabian StyleLiu, Bin, Ming Tang, and Hui Chen. 2022. "Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 CYP4BQ1 Responsible for (+)-α-Pinene Tolerance in Dendroctonus armandi" International Journal of Molecular Sciences 23, no. 19: 11578. https://doi.org/10.3390/ijms231911578