Sox6 Differentially Regulates Inherited Myogenic Abilities and Muscle Fiber Types of Satellite Cells Derived from Fast- and Slow-Type Muscles
Abstract
:1. Introduction
2. Results
2.1. Different Myogenic Potentials in Satellite Cells of Distinct Muscle Fiber Types
2.2. Sox6 Promotes the Myogenesis and Development of Satellite Cells Derived from Fast Type-Enriched Pectoral Muscles towards Intrinsic Tendency
2.3. Different Roles of Sox6 in Satellite Cells Derived from Slow Type-Enriched Leg Muscles
2.4. Differential Expression of Nfix between LM-MBs and PM-MBs at the Differentiation Stage Leads to Different Roles of Sox6 in the Regulation of Slow-Type Fibers
2.5. Sox6 Indirectlyupregulates the Slow-Type Isoforms through the Activation of Mef2C
2.6. Sox6 Decreases Slow-Type Isoforms through Binding with Nfix in PM-MTs or Increases Slow-Type Isoforms by Activating Mef2C in LM-MTs
3. Discussion
4. Materials and Methods
4.1. Ethics Standards
4.2. Isolation of Muscle Satellite Cells and Cell Culture
4.3. Immunofluorescence
4.4. Edu and Flow Cytometry Assays
4.5. RNA Extraction, cDNA Synthesis and Quantitative Real-Time PCR
4.6. Plasmid Construction, RNA Oligonucleotides and Cell Transfection
4.7. Western Blot
4.8. Dual-Luciferase Reporter Assay
4.9. Co-Immunoprecipitation Assay
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, Z.; Lin, S.; Luo, W.; Ren, T.; Huang, X.; Li, W.; Zhang, X. Sox6 Differentially Regulates Inherited Myogenic Abilities and Muscle Fiber Types of Satellite Cells Derived from Fast- and Slow-Type Muscles. Int. J. Mol. Sci. 2022, 23, 11327. https://doi.org/10.3390/ijms231911327
Zhang Z, Lin S, Luo W, Ren T, Huang X, Li W, Zhang X. Sox6 Differentially Regulates Inherited Myogenic Abilities and Muscle Fiber Types of Satellite Cells Derived from Fast- and Slow-Type Muscles. International Journal of Molecular Sciences. 2022; 23(19):11327. https://doi.org/10.3390/ijms231911327
Chicago/Turabian StyleZhang, Zihao, Shudai Lin, Wen Luo, Tuanhui Ren, Xing Huang, Wangyu Li, and Xiquan Zhang. 2022. "Sox6 Differentially Regulates Inherited Myogenic Abilities and Muscle Fiber Types of Satellite Cells Derived from Fast- and Slow-Type Muscles" International Journal of Molecular Sciences 23, no. 19: 11327. https://doi.org/10.3390/ijms231911327