The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat
Abstract
:1. Introduction
2. Results
2.1. TaCOPT3D Expression in Wheat under Cadmium Stress
2.2. Subcellular Localization of TaCOPT3D
2.3. Overexpression of TaCOPT3D in Wheat Increased Cd Tolerance
2.4. Overexpression of TaCOPT3D Enhanced the Antioxidant Capacity in Transgenic Wheat under Cd Stress
2.5. TaCPOT3D Influences the Cd2+ Flux in Wheat Roots
2.6. TaWRKY22 Binds to TaCPOT3D Promoter
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Treatments
4.2. Cloning of Coding Sequence of TaCOPT3D
4.3. Analysis of Gene Expression
4.4. Gene Cloning and Plant Transformation
4.5. Detection of Antioxidant Enzyme Activity
4.6. Measurements of Cd2+ Flux
4.7. Measurement of Cd Contents
4.8. Yeast One-Hybrid (Y1H) Assay
4.9. Electrophoretic Mobility Shift Assay (EMSA)
4.10. Transient Expression Assays in Tobacco Leaves
4.11. Statistical Analysis
4.12. Primers
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, X.; Wang, H.; He, F.; Du, X.; Ren, M.; Bao, Y. The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat. Int. J. Mol. Sci. 2022, 23, 10379. https://doi.org/10.3390/ijms231810379
Liu X, Wang H, He F, Du X, Ren M, Bao Y. The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat. International Journal of Molecular Sciences. 2022; 23(18):10379. https://doi.org/10.3390/ijms231810379
Chicago/Turabian StyleLiu, Xiaojuan, Hongcheng Wang, Fang He, Xuye Du, Mingjian Ren, and Yinguang Bao. 2022. "The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat" International Journal of Molecular Sciences 23, no. 18: 10379. https://doi.org/10.3390/ijms231810379