Pharmacological Screening of Venoms from Five Brazilian Micrurus Species on Different Ion Channels
Abstract
:1. Introduction
2. Results
2.1. Chromatographic Profile of Five Micrurus Venoms from the South and Southeast Brazil
2.2. Micrurus Venoms Are Not Cytotoxic to X. laevis Oocytes
2.3. Micrurus Frontalis Venom Evoked Cl− Outward Current by Acting Directly or Indirectly on Ca2+-Activated Cl− Channels
2.4. Micrurus Venoms Act in Different Ion Channels
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Venoms
5.2. RP-HPLC Profile
5.3. Electrophysiologic Recordings on Xenopus laevis Oocytes
5.3.1. Cytotoxic Experiments with Non-Injected Oocytes
5.3.2. Niflumic Acid Assay
5.3.3. Ion Channels Heterologous Expression and Recordings in Oocytes
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kleiz-Ferreira, J.M.; Bernaerts, H.; Pinheiro-Junior, E.L.; Peigneur, S.; Zingali, R.B.; Tytgat, J. Pharmacological Screening of Venoms from Five Brazilian Micrurus Species on Different Ion Channels. Int. J. Mol. Sci. 2022, 23, 7714. https://doi.org/10.3390/ijms23147714
Kleiz-Ferreira JM, Bernaerts H, Pinheiro-Junior EL, Peigneur S, Zingali RB, Tytgat J. Pharmacological Screening of Venoms from Five Brazilian Micrurus Species on Different Ion Channels. International Journal of Molecular Sciences. 2022; 23(14):7714. https://doi.org/10.3390/ijms23147714
Chicago/Turabian StyleKleiz-Ferreira, Jessica Matos, Hans Bernaerts, Ernesto Lopes Pinheiro-Junior, Steve Peigneur, Russolina Benedeta Zingali, and Jan Tytgat. 2022. "Pharmacological Screening of Venoms from Five Brazilian Micrurus Species on Different Ion Channels" International Journal of Molecular Sciences 23, no. 14: 7714. https://doi.org/10.3390/ijms23147714