Genome-Wide Identification of TaSAUR Gene Family Members in Hexaploid Wheat and Functional Characterization of TaSAUR66-5B in Improving Nitrogen Use Efficiency
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification and Characterization of TaSAUR Family Members in Wheat
2.2. Phylogenetic Relationships of SAURs in Five Species
2.3. Gene Structure and Conserved Motifs of TaSAURs
2.4. Cis-Elements in the Promoters of TaSAURs
2.5. Gene Expression Analysis of TaSAURs under Exogenous Hormones Treatment
2.6. Functional Analysis of TaSAUR66-5B
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Identification and Classification the TaSAUR Members in Wheat
4.3. Characterization of Predicted TaSAUR Proteins
4.4. Analysis of Conserved TaSAUR Motifs and Gene Structures
4.5. Phylogenetic Analysis
4.6. Analysis of Cis-Elements
4.7. Growth Conditions and Treatments in the Hydroponic Culture
4.8. Field Experiment
4.9. Real-Time Quantitative PCR and Data Analysis
4.10. Statistical Analysis of Data
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lv, W.; He, X.; Guo, H.; Lan, H.; Jiao, Y.; Li, L.; Lian, Y.; Wang, Z.; Xin, Z.; Ren, Y.; et al. Genome-Wide Identification of TaSAUR Gene Family Members in Hexaploid Wheat and Functional Characterization of TaSAUR66-5B in Improving Nitrogen Use Efficiency. Int. J. Mol. Sci. 2022, 23, 7574. https://doi.org/10.3390/ijms23147574
Lv W, He X, Guo H, Lan H, Jiao Y, Li L, Lian Y, Wang Z, Xin Z, Ren Y, et al. Genome-Wide Identification of TaSAUR Gene Family Members in Hexaploid Wheat and Functional Characterization of TaSAUR66-5B in Improving Nitrogen Use Efficiency. International Journal of Molecular Sciences. 2022; 23(14):7574. https://doi.org/10.3390/ijms23147574
Chicago/Turabian StyleLv, Weizeng, Xue He, Haojuan Guo, Haibin Lan, Yanqing Jiao, Le Li, Yanhao Lian, Zhiqiang Wang, Zeyu Xin, Yongzhe Ren, and et al. 2022. "Genome-Wide Identification of TaSAUR Gene Family Members in Hexaploid Wheat and Functional Characterization of TaSAUR66-5B in Improving Nitrogen Use Efficiency" International Journal of Molecular Sciences 23, no. 14: 7574. https://doi.org/10.3390/ijms23147574