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Volume 23
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10.3390/ijms23147492
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Article

The MexJK Multidrug Efflux Pump Is Not Involved in Acquired or Intrinsic Antibiotic Resistance in Pseudomonas aeruginosa, but Modulates the Bacterial Quorum Sensing Response

by
Rafael Amieva
1,2,
Teresa Gil-Gil
1,3,
José Luis Martínez
1,* and
Manuel Alcalde-Rico
1,4,5,*
1
Centro Nacional de Biotecnología, CSIC, Darwin 3, 28049 Madrid, Spain
2
SALUVET Group, Animal Health Department, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain
3
Programa de Doctorado en Biociencias Moleculares, Universidad Autónoma de Madrid, 28049 Madrid, Spain
4
Grupo de Resistencia Antimicrobiana en Bacterias Patógenas y Ambientales (GRABPA), Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile
5
Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 7550000, Chile
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(14), 7492; https://doi.org/10.3390/ijms23147492
Submission received: 7 June 2022 / Revised: 1 July 2022 / Accepted: 2 July 2022 / Published: 6 July 2022
(This article belongs to the Section Molecular Microbiology)
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Abstract

Multidrug efflux pumps are critical elements in both intrinsic and acquired antibiotic resistance of bacterial populations. Consequently, most studies regarding these protein machineries focus on this specific phenotype. Nevertheless, different works show that efflux pumps participate in other aspects of bacterial physiology too. Herein, we study the Pseudomonas aeruginosa multidrug efflux pump MexJK. Previous studies, using model strains lacking MexAB-OprM and MexCD-OprJ efflux pumps, support that MexJK can extrude erythromycin, tetracycline, and triclosan. However, the results here reported indicate that this potential increased extrusion, in a mutant overexpressing mexJK, does not alter the antibiotics susceptibility in a wild-type genetic background where all intrinsic multidrug efflux pumps remain functional. Nevertheless, a clear impact on the quorum sensing (QS) response, mainly in the Pqs-dependent QS regulation network and in the expression of Pqs-regulated virulence factors, was observed linked to mexJK overexpression. The production of the siderophore pyoverdine strongly depended on the level of mexJK expression, suggesting that MexJK might participate in P. aeruginosa pyoverdine-dependent iron homeostasis. All in all, the results presented in the current article support that the functions of multidrug efflux pumps, as MexJK, go beyond antibiotic resistance and can modulate other relevant aspects of bacterial physiology.
Keywords: Pseudomonas aeruginosa; quorum sensing; antibiotic resistance; PQS; pyoverdine; MexJK Pseudomonas aeruginosa; quorum sensing; antibiotic resistance; PQS; pyoverdine; MexJK

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MDPI and ACS Style

Amieva, R.; Gil-Gil, T.; Martínez, J.L.; Alcalde-Rico, M. The MexJK Multidrug Efflux Pump Is Not Involved in Acquired or Intrinsic Antibiotic Resistance in Pseudomonas aeruginosa, but Modulates the Bacterial Quorum Sensing Response. Int. J. Mol. Sci. 2022, 23, 7492. https://doi.org/10.3390/ijms23147492

AMA Style

Amieva R, Gil-Gil T, Martínez JL, Alcalde-Rico M. The MexJK Multidrug Efflux Pump Is Not Involved in Acquired or Intrinsic Antibiotic Resistance in Pseudomonas aeruginosa, but Modulates the Bacterial Quorum Sensing Response. International Journal of Molecular Sciences. 2022; 23(14):7492. https://doi.org/10.3390/ijms23147492

Chicago/Turabian Style

Amieva, Rafael, Teresa Gil-Gil, José Luis Martínez, and Manuel Alcalde-Rico. 2022. "The MexJK Multidrug Efflux Pump Is Not Involved in Acquired or Intrinsic Antibiotic Resistance in Pseudomonas aeruginosa, but Modulates the Bacterial Quorum Sensing Response" International Journal of Molecular Sciences 23, no. 14: 7492. https://doi.org/10.3390/ijms23147492

APA Style

Amieva, R., Gil-Gil, T., Martínez, J. L., & Alcalde-Rico, M. (2022). The MexJK Multidrug Efflux Pump Is Not Involved in Acquired or Intrinsic Antibiotic Resistance in Pseudomonas aeruginosa, but Modulates the Bacterial Quorum Sensing Response. International Journal of Molecular Sciences, 23(14), 7492. https://doi.org/10.3390/ijms23147492

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