Next Article in Journal
Molecular Mechanism of Equine Endometrosis: The NF-κB-Dependent Pathway Underlies the Ovarian Steroid Receptors’ Dysfunction
Next Article in Special Issue
Activation of Humoral Immunity during the Pathogenesis of Experimental Chronic Lung Allograft Dysfunction
Previous Article in Journal
Alternative Splicing and Its Roles in Plant Metabolism
 
 
Article

Combined Analysis of HLA Class II Eplet Mismatch and Tacrolimus Levels for the Prediction of De Novo Donor Specific Antibody Development in Kidney Transplant Recipients

1
Department of Laboratory Medicine, International St. Mary’s Hospital, College of Medicine, Catholic Kwandong University, Incheon 22711, Korea
2
Division of Nephrology, Department of Internal Medicine, Bucheon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Bucheon 14647, Korea
3
Transplantation Research Center, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
4
Department of Laboratory Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
5
Division of Nephrology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
6
Division of Nephrology, Department of Internal Medicine, Incheon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
7
Division of Nephrology, Department of Internal Medicine, Konyang University Hospital, College of Medicine, Konyang University, Daejeon 35365, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Mihai Oltean
Int. J. Mol. Sci. 2022, 23(13), 7357; https://doi.org/10.3390/ijms23137357
Received: 27 May 2022 / Revised: 29 June 2022 / Accepted: 29 June 2022 / Published: 1 July 2022
(This article belongs to the Special Issue Allograft Rejection: Emerging Molecular Mechanisms and Biomarkers)
We investigated whether HLA class II eplet mismatch was related to dnDSA development and analyzed its combined impact with tacrolimus levels for kidney transplantation outcomes. A total of 347 kidney transplants were included. HLA Matchmaker was used for the single molecular eplet, total eplet, antibody (Ab)-verified eplet mismatch analyses, and Ab-verified single molecular analysis to identify HLA-DR/DQ molecular thresholds for the risk of dnDSA development. A time-weighted tacrolimus trough level (TAC-C0) of 5 ng/mL and a TAC-C0 time-weighted coefficient variability (TWCV) of 20% were applied to find the combined effects on dnDSA development. A high level of mismatch for single molecular eplet (DQ ≥ 10), total eplet (DQ ≥ 12), Ab-verified eplet (DQ ≥ 4), and Ab-verified single molecular eplet (DQ ≥ 4) significantly correlated with HLA class II dnDSA development. Class II dnDSA developed mostly in patients with low TAC-C0 and high eplet mismatch. In the multivariable analyses, low TAC-C0 and high eplet mismatch showed the highest hazard ratio for the development of dnDSA. No significant combined effect was observed in dnDSA development according to TWCV. In conclusion, the determination of HLA class II eplet mismatch may improve the risk stratification for dnDSA development, especially in conjunction with tacrolimus trough levels. View Full-Text
Keywords: eplet; donor specific antibody; kidney transplantation; tacrolimus trough level eplet; donor specific antibody; kidney transplantation; tacrolimus trough level
Show Figures

Figure 1

MDPI and ACS Style

Lee, H.; Min, J.W.; Kang, H.; Lee, H.; Eum, S.H.; Park, Y.; Yang, C.W.; Chung, B.H.; Oh, E.-J. Combined Analysis of HLA Class II Eplet Mismatch and Tacrolimus Levels for the Prediction of De Novo Donor Specific Antibody Development in Kidney Transplant Recipients. Int. J. Mol. Sci. 2022, 23, 7357. https://doi.org/10.3390/ijms23137357

AMA Style

Lee H, Min JW, Kang H, Lee H, Eum SH, Park Y, Yang CW, Chung BH, Oh E-J. Combined Analysis of HLA Class II Eplet Mismatch and Tacrolimus Levels for the Prediction of De Novo Donor Specific Antibody Development in Kidney Transplant Recipients. International Journal of Molecular Sciences. 2022; 23(13):7357. https://doi.org/10.3390/ijms23137357

Chicago/Turabian Style

Lee, Hyeyoung, Ji Won Min, Hyunhye Kang, Hanbi Lee, Sang Hun Eum, Yohan Park, Chul Woo Yang, Byung Ha Chung, and Eun-Jee Oh. 2022. "Combined Analysis of HLA Class II Eplet Mismatch and Tacrolimus Levels for the Prediction of De Novo Donor Specific Antibody Development in Kidney Transplant Recipients" International Journal of Molecular Sciences 23, no. 13: 7357. https://doi.org/10.3390/ijms23137357

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop