Antibiofilm Activities of Cinnamaldehyde Analogs against Uropathogenic Escherichia coli and Staphylococcus aureus
Abstract
:1. Introduction
2. Results
2.1. Antibiofilm and Antimicrobial Activities of Trans-CNMA and Its Derivatives against UPEC and S. aureus
2.2. Microscopic Observations of Biofilm Formation by UPEC and S. aureus
2.3. Trans-CNMA and 4-nitroCNMA Affected the Swimming Motility of UPEC
2.4. Effects of Trans-CNMA and 4-nitroCNMA on Cell Hydrophobicity and Hemolysis
2.5. Antibiofilm Activities of CNMAs against Mixed UPEC and S. aureus Biofilms
3. Discussion
4. Materials and Methods
4.1. Reagents and Culture Strains
4.2. Crystal Violet Biofilm Assay
4.3. Microscopic Observations of Biofilms
4.4. Swimming Motilities of UPEC
4.5. Cell Surface Hydrophobicities
4.6. Staphyloxanthin Assay
4.7. Sheep Red Blood Cell Hemolysis Assay
4.8. The Mixed UPEC/S. aureus Biofilm Model
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Test Compound | Structure | MIC (µg/mL) against UPEC | MIC (µg/mL) against S. aureus |
---|---|---|---|
Trans-CNMA | 400 | >400 | |
4-BromoCNMA | 400 | >400 | |
4-ChloroCNMA | 200 | 400 | |
CNMA oxime | >400 | >400 | |
4-DimethylaminoCNMA | >400 | >400 | |
4-FluoroCNMA | 200 | >400 | |
2-MethoxyCNMA | >400 | >400 | |
4-MethoxyCNMA | >400 | >400 | |
α-MethylCNMA | >400 | >400 | |
2-NitroCNMA | >400 | >400 | |
4-NitroCNMA | 100 | 100 |
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Kim, Y.; Kim, S.; Cho, K.-H.; Lee, J.-H.; Lee, J. Antibiofilm Activities of Cinnamaldehyde Analogs against Uropathogenic Escherichia coli and Staphylococcus aureus. Int. J. Mol. Sci. 2022, 23, 7225. https://doi.org/10.3390/ijms23137225
Kim Y, Kim S, Cho K-H, Lee J-H, Lee J. Antibiofilm Activities of Cinnamaldehyde Analogs against Uropathogenic Escherichia coli and Staphylococcus aureus. International Journal of Molecular Sciences. 2022; 23(13):7225. https://doi.org/10.3390/ijms23137225
Chicago/Turabian StyleKim, Yeseul, Sanghun Kim, Kiu-Hyung Cho, Jin-Hyung Lee, and Jintae Lee. 2022. "Antibiofilm Activities of Cinnamaldehyde Analogs against Uropathogenic Escherichia coli and Staphylococcus aureus" International Journal of Molecular Sciences 23, no. 13: 7225. https://doi.org/10.3390/ijms23137225