Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans
Abstract
:1. Introduction
2. Results
2.1. nse-4 Mutants Exhibited Reduced Fecundity and Chromosome Defect at Diakinesis
2.2. Delayed Development in nse-4 Mutants Is Exacerbated by Genotoxic Stress
2.3. nse-4 Is Required for Efficient DNA Repair in Germ Cells
2.4. nse-4 Deficiency Led to Increase RAD-51 Accumulation
2.5. nse-4 Mutants Are Defective in Inter-Sister Repair
2.6. Apoptosis Increased in the nse-4 Deficient Germ Cells
2.7. NSE-4 Localizes on the Chromosome and Is Indispensable for the Localization of NSE-1
3. Discussion
4. Materials and Methods
4.1. Worm Strains and Growth Condition
4.2. Phenotypic Assays (Brood-Size, Progeny Viability and Male Frequency)
4.3. Genotoxic Treatment and Animal Developmental Scoring
4.4. Genotoxic Treatment and Progeny Viability Scoring
4.5. Worm Total RNA Extraction and RT-qPCR
4.6. Construction of gfp::3xflag::nse-4 Strain and Microinjection
4.7. Cytological Preparations and Staining
4.8. Microscopy Procedures
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Odiba, A.S.; Ezechukwu, C.S.; Liao, G.; Li, S.; Chen, Z.; Liu, X.; Fang, W.; Jin, C.; Wang, B. Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans. Int. J. Mol. Sci. 2022, 23, 7202. https://doi.org/10.3390/ijms23137202
Odiba AS, Ezechukwu CS, Liao G, Li S, Chen Z, Liu X, Fang W, Jin C, Wang B. Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans. International Journal of Molecular Sciences. 2022; 23(13):7202. https://doi.org/10.3390/ijms23137202
Chicago/Turabian StyleOdiba, Arome Solomon, Chiemekam Samuel Ezechukwu, Guiyan Liao, Siqiao Li, Zhongliang Chen, Xihui Liu, Wenxia Fang, Cheng Jin, and Bin Wang. 2022. "Loss of NSE-4 Perturbs Genome Stability and DNA Repair in Caenorhabditis elegans" International Journal of Molecular Sciences 23, no. 13: 7202. https://doi.org/10.3390/ijms23137202