Next Article in Journal
Recent Advances in Molecular Research on Hydrogen Sulfide (H2S) Role in Diabetes Mellitus (DM)—A Systematic Review
Previous Article in Journal
New Borane-Protected Derivatives of α-Aminophosphonous Acid as Anti-Osteosarcoma Agents: ADME Analysis and Molecular Modeling, In Vitro Studies on Anti-Cancer Activities, and NEP Inhibition as a Possible Mechanism of Anti-Proliferative Activity
 
 
Article

Effect of Psilocybin and Ketamine on Brain Neurotransmitters, Glutamate Receptors, DNA and Rat Behavior

1
Department of Pharmacology, Unit II, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343 Kraków, Poland
2
Department of Pharmacology, Laboratory of Pharmacology and Brain Biostructure, Maj Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343 Kraków, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Philippe De Deurwaerdere and Franck Vandermoere
Int. J. Mol. Sci. 2022, 23(12), 6713; https://doi.org/10.3390/ijms23126713
Received: 28 May 2022 / Revised: 14 June 2022 / Accepted: 14 June 2022 / Published: 16 June 2022
(This article belongs to the Special Issue Psychedelic Drugs and Beyond)
Clinical studies provide evidence that ketamine and psilocybin could be used as fast-acting antidepressants, though their mechanisms and toxicity are still not fully understood. To address this issue, we have examined the effect of a single administration of ketamine and psilocybin on the extracellular levels of neurotransmitters in the rat frontal cortex and reticular nucleus of the thalamus using microdialysis. The genotoxic effect and density of glutamate receptor proteins was measured with comet assay and Western blot, respectively. An open field test, light–dark box test and forced swim test were conducted to examine rat behavior 24 h after drug administration. Ketamine (10 mg/kg) and psilocybin (2 and 10 mg/kg) increased dopamine, serotonin, glutamate and GABA extracellular levels in the frontal cortex, while psilocybin also increased GABA in the reticular nucleus of the thalamus. Oxidative DNA damage due to psilocybin was observed in the frontal cortex and from both drugs in the hippocampus. NR2A subunit levels were increased after psilocybin (10 mg/kg). Behavioral tests showed no antidepressant or anxiolytic effects, and only ketamine suppressed rat locomotor activity. The observed changes in neurotransmission might lead to genotoxicity and increased NR2A levels, while not markedly affecting animal behavior. View Full-Text
Keywords: dopamine; serotonin; glutamate; GABA; microdialysis; DNA damage; glutamate receptors; light–dark box test; open field test; forced swim test dopamine; serotonin; glutamate; GABA; microdialysis; DNA damage; glutamate receptors; light–dark box test; open field test; forced swim test
Show Figures

Figure 1

MDPI and ACS Style

Wojtas, A.; Bysiek, A.; Wawrzczak-Bargiela, A.; Szych, Z.; Majcher-Maślanka, I.; Herian, M.; Maćkowiak, M.; Gołembiowska, K. Effect of Psilocybin and Ketamine on Brain Neurotransmitters, Glutamate Receptors, DNA and Rat Behavior. Int. J. Mol. Sci. 2022, 23, 6713. https://doi.org/10.3390/ijms23126713

AMA Style

Wojtas A, Bysiek A, Wawrzczak-Bargiela A, Szych Z, Majcher-Maślanka I, Herian M, Maćkowiak M, Gołembiowska K. Effect of Psilocybin and Ketamine on Brain Neurotransmitters, Glutamate Receptors, DNA and Rat Behavior. International Journal of Molecular Sciences. 2022; 23(12):6713. https://doi.org/10.3390/ijms23126713

Chicago/Turabian Style

Wojtas, Adam, Agnieszka Bysiek, Agnieszka Wawrzczak-Bargiela, Zuzanna Szych, Iwona Majcher-Maślanka, Monika Herian, Marzena Maćkowiak, and Krystyna Gołembiowska. 2022. "Effect of Psilocybin and Ketamine on Brain Neurotransmitters, Glutamate Receptors, DNA and Rat Behavior" International Journal of Molecular Sciences 23, no. 12: 6713. https://doi.org/10.3390/ijms23126713

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop