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Article

Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus

by 1, 1, 1,2, 2,3 and 1,2,3,*
1
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
3
The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China
*
Author to whom correspondence should be addressed.
Academic Editors: Gad Degani and Jan Kaslin
Int. J. Mol. Sci. 2022, 23(11), 5861; https://doi.org/10.3390/ijms23115861
Received: 12 April 2022 / Revised: 22 May 2022 / Accepted: 22 May 2022 / Published: 24 May 2022
(This article belongs to the Special Issue Molecular Sexual Determination and Differentiation in Fishes)
Germline stem cells (GSCs) are a group of unique adult stem cells in gonads that act as important transmitters for genetic information. Donor GSCs have been used to produce offspring by transplantation in fisheries. In this study, we successfully isolated and enriched GSCs from the ovary, ovotestis, and testis of Monopterus albus, one of the most important breeding freshwater fishes in China. Transcriptome comparison assay suggests that a distinct molecular signature exists in each type of GSC, and that different signaling activities are required for the maintenance of distinct GSCs. Functional analysis shows that fGSCs can successfully colonize and contribute to the germline cell lineage of a host zebrafish gonad after transplantation. Finally, we describe a simple feeder-free method for the isolation and enrichment of GSCs that can contribute to the germline cell lineage of zebrafish embryos and generate the germline chimeras after transplantation. View Full-Text
Keywords: the swamp eel; germline stem cells; ovary; testis; ovotestis; transplantation the swamp eel; germline stem cells; ovary; testis; ovotestis; transplantation
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MDPI and ACS Style

Sun, X.; Tao, B.; Wang, Y.; Hu, W.; Sun, Y. Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus. Int. J. Mol. Sci. 2022, 23, 5861. https://doi.org/10.3390/ijms23115861

AMA Style

Sun X, Tao B, Wang Y, Hu W, Sun Y. Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus. International Journal of Molecular Sciences. 2022; 23(11):5861. https://doi.org/10.3390/ijms23115861

Chicago/Turabian Style

Sun, Xiaoyun, Binbin Tao, Yongxin Wang, Wei Hu, and Yuhua Sun. 2022. "Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus" International Journal of Molecular Sciences 23, no. 11: 5861. https://doi.org/10.3390/ijms23115861

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