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Article

Identification of an Epigenetically Marked Locus within the Sex Determination Region of Channel Catfish

1
The Fish Molecular Genetics and Biotechnology Laboratory, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA
2
Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China
3
Department of Biology, College of Arts and Sciences, Syracuse University, Syracuse, NY 13244, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Gad Degani
Int. J. Mol. Sci. 2022, 23(10), 5471; https://doi.org/10.3390/ijms23105471 (registering DOI)
Received: 8 April 2022 / Revised: 6 May 2022 / Accepted: 11 May 2022 / Published: 13 May 2022
(This article belongs to the Special Issue Molecular Sexual Determination and Differentiation in Fishes)
Channel catfish has an XY sex determination system. However, the X and Y chromosomes harbor an identical gene content of 950 genes each. In this study, we conducted comparative analyses of methylome and transcriptome of genetic males and genetic females before gonadal differentiation to provide insights into the mechanisms of sex determination. Differentially methylated CpG sites (DMCs) were predominantly identified on the sex chromosome, most notably within the sex determination region (SDR), although the overall methylation profiles across the entire genome were similar between genetic males and females. The drastic differences in methylation were located within the SDR at nucleotide position 14.0–20.3 Mb of the sex chromosome, making this region an epigenetically marked locus within the sex determination region. Most of the differentially methylated CpG sites were hypermethylated in females and hypomethylated in males, suggesting potential involvement of methylation modification in sex determination in channel catfish. Along with the differential methylation in the SDR, a number of differentially expressed genes within the SDR were also identified between genetic males and females, making them potential candidate genes for sex determination and differentiation in channel catfish. View Full-Text
Keywords: fish; sex determination; methylation; transcriptome; epigenetics; RNA-Seq; sex differentiation fish; sex determination; methylation; transcriptome; epigenetics; RNA-Seq; sex differentiation
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MDPI and ACS Style

Yang, Y.; Zhou, T.; Liu, Y.; Tian, C.; Bao, L.; Wang, W.; Zhang, Y.; Liu, S.; Shi, H.; Tan, S.; Gao, D.; Dunham, R.A.; Liu, Z. Identification of an Epigenetically Marked Locus within the Sex Determination Region of Channel Catfish. Int. J. Mol. Sci. 2022, 23, 5471. https://doi.org/10.3390/ijms23105471

AMA Style

Yang Y, Zhou T, Liu Y, Tian C, Bao L, Wang W, Zhang Y, Liu S, Shi H, Tan S, Gao D, Dunham RA, Liu Z. Identification of an Epigenetically Marked Locus within the Sex Determination Region of Channel Catfish. International Journal of Molecular Sciences. 2022; 23(10):5471. https://doi.org/10.3390/ijms23105471

Chicago/Turabian Style

Yang, Yujia, Tao Zhou, Yang Liu, Changxu Tian, Lisui Bao, Wenwen Wang, Yu Zhang, Shikai Liu, Huitong Shi, Suxu Tan, Dongya Gao, Rex A. Dunham, and Zhanjiang Liu. 2022. "Identification of an Epigenetically Marked Locus within the Sex Determination Region of Channel Catfish" International Journal of Molecular Sciences 23, no. 10: 5471. https://doi.org/10.3390/ijms23105471

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