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Article

Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells

1
Stem Cell Research Centre, Department of Hematology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow 226014, India
2
Indian Institute of Science (IISC), Bangalore 560012, India
3
ICMR-National Institute for Research in Environmental Health, Bhopal 462030, India
4
Institute of Clinical and Preventive Medicine, University of Latvia, LV-1586 Riga, Latvia
5
Faculty of Medicine, University of Latvia, LV-1586 Riga, Latvia
6
Riga East University Hospital, LV-1038 Riga, Latvia
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Yun-Wen Zheng
Int. J. Mol. Sci. 2022, 23(10), 5816; https://doi.org/10.3390/ijms23105816
Received: 5 May 2022 / Revised: 20 May 2022 / Accepted: 20 May 2022 / Published: 22 May 2022
Mesenchymal stem cell (MSC)-based therapy and tissue repair necessitate the use of an ideal clinical biomaterial capable of increasing cell proliferation and differentiation. Recently, MXenes 2D nanomaterials have shown remarkable potential for improving the functional properties of MSCs. In the present study, we elucidated the potential of Ti2CTx MXene as a biomaterial through its primary biological response to human Wharton’s Jelly MSCs (hWJ-MSCs). A Ti2CTx nanosheet was synthesized and thoroughly characterized using various microscopic and spectroscopic tools. Our findings suggest that Ti2CTx MXene nanosheet exposure does not alter the morphology of the hWJ-MSCs; however, it causes a dose-dependent (10–200 µg/mL) increase in cell proliferation, and upon using it with conditional media, it also enhanced its tri-lineage differentiation potential, which is a novel finding of our study. A two-fold increase in cell viability was also noticed at the highest tested dose of the nanosheet. The treated hWJ-MSCs showed no sign of cellular stress or toxicity. Taken together, these findings suggest that the Ti2CTx MXene nanosheet is capable of augmenting the proliferation and differentiation potential of the cells. View Full-Text
Keywords: Ti2CTx MXene; nanosheets; hWJ-MSCs; cell proliferation; cell viability; stemness; cell differentiation Ti2CTx MXene; nanosheets; hWJ-MSCs; cell proliferation; cell viability; stemness; cell differentiation
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MDPI and ACS Style

Singh, S.K.; Singh, A.; Kumar, V.; Gupta, J.; Umrao, S.; Kumar, M.; Sarma, D.K.; Leja, M.; Bhandari, M.P.; Verma, V. Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells. Int. J. Mol. Sci. 2022, 23, 5816. https://doi.org/10.3390/ijms23105816

AMA Style

Singh SK, Singh A, Kumar V, Gupta J, Umrao S, Kumar M, Sarma DK, Leja M, Bhandari MP, Verma V. Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2022; 23(10):5816. https://doi.org/10.3390/ijms23105816

Chicago/Turabian Style

Singh, Suraj Kumar, Anshuman Singh, Vinod Kumar, Jalaj Gupta, Sima Umrao, Manoj Kumar, Devojit Kumar Sarma, Marcis Leja, Manohar Prasad Bhandari, and Vinod Verma. 2022. "Nanosheets Based Approach to Elevate the Proliferative and Differentiation Efficacy of Human Wharton’s Jelly Mesenchymal Stem Cells" International Journal of Molecular Sciences 23, no. 10: 5816. https://doi.org/10.3390/ijms23105816

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